基因剔除小鼠
基因敲除
肝细胞
条件基因敲除
脂质代谢
代谢组学
免疫系统
生物
细胞生物学
免疫学
内分泌学
生物信息学
表型
基因
生物化学
体外
作者
Quancheng Cheng,Junwei Zhang,Huiru Ding,Ziyuan Wang,Jinyu Fang,Xuan Fang,Man Li,Rui Li,Jieyi Meng,Huaicun Liu,Xin Lü,Yiyao Xu,Chunhua Chen,Weiguang Zhang
标识
DOI:10.1016/j.ijbiomac.2023.125459
摘要
Aquaporin 9 (AQP9) is the main channel by which blood glycerol enters the liver, where it plays key roles in osmotic pressure regulation and energy metabolism. Previous studies have shown that AQP9 is involved in the pathogenesis of many liver diseases. In this study, we aimed to clarify the role of AQP9 in maintaining the physiological environment of the liver using Aqp9-/- mice. We constructed Aqp9 knockout mice and used comprehensive multiomics analysis to elucidate the potential molecular effects of AQP9 expression on liver tissue. Knockout of Aqp9 reduced mouse body weight by affecting glycerol metabolism and led to hepatocyte death and inflammatory cell infiltration, which was confirmed by transcriptomics, proteomics and metabolomics. Moreover, knockout of Aqp9 triggered immune and inflammatory responses, leading to scattered and mild liver cell pyroptosis and compensatory liver cell proliferation.
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