Transcription factor NFE2L3 promotes the proliferation of esophageal squamous cell carcinoma cells and causes radiotherapy resistance by regulating IL-6

辐射敏感性 克隆形成试验 抗辐射性 生物 细胞培养 转染 细胞生长 分子生物学 流式细胞术 癌症研究 医学 放射治疗 遗传学 内科学
作者
Tingting Chen,Bing Xu,Hui Chen,Yuanyuan Sun,Jiahang Song,Xinchen Sun,Xizhi Zhang,Hua Wei
出处
期刊:Computer Methods and Programs in Biomedicine [Elsevier]
卷期号:226: 107102-107102 被引量:2
标识
DOI:10.1016/j.cmpb.2022.107102
摘要

To scrutinize the impact of overexpression and interference of NFE2L3 on radiosensitivity of esophageal squamous cell carcinoma cells (ESCC) and its downstream mechanism and to assess whether NFE2L3 expression alters in vivo radiosensitivity of ESCC by developing a subcutaneous tumor model in mice.Through RNA-Seq, we compared the differentially expressed genes between the ECA-109R cell line and its parent ECA-109 cell line. The differentially expressed genes were selected and verified by qRT-PCR. Transfection of ESCC cell lines with NFE2L3 inhibitor or mimic lentivirus constructs was done to study the activity of NFE2L3. To assess the effect of NFE2L3 on cellular growth and proliferation, clonogenic survival assay, EdU incorporation assay, and CCK-8 assay were done after irradiation. To probe how many irradiated DNA double-strand breaks were produced, the corresponding intensity of γ-H2AX foci were detected by immunofluorescence. Apoptotic cells were assayed by flow cytometry assay after irradiation; To investigate the downstream genes of NFE2L3, we knocked NFE2L3, and RNA-Seq was used to find out the downstream genes. qRT-PCR and western blot ensued to score associated protein profiles. The in vivo ESCC cell radiosensitivity was scrutinized by nude mouse xenograft models.The differential genes between ECA-109R cells and its parent ECA-109 cells were compared by qRT-PCR to unveil a significant increase in NFE2L3 expression. Functional analysis indicated that NFE2L3 increased radioresistance in ESCC cells. Then, through high-throughput sequencing and bioinformatics analysis, IL-6 was found to be a hub gene that played a role downstream of NFE2L3 and was verified by qRT-PCR, western blot, and double luciferase reporter gene experiment. NFE2L3 could regulate ESCC cell radiosensitivity via the IL-6-STAT3 signaling pathway, and downregulation of IL-6 expression could reverse the effects of highly expressed NFE2L3. In vivo tumor xenograft experiments confirmed that NFE2L3 affects the sensitivity to radiation therapy.NFE2L3 can affect the radiosensitivity of ESCC cells through IL-6 transcription and IL-6/STAT3 signaling pathway. This makes NFE2L3 a putative target to regulate ESCC cell radiosensitivity.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
yhr发布了新的文献求助10
刚刚
刚刚
月亮完成签到 ,获得积分10
1秒前
Ronggaz发布了新的文献求助10
1秒前
1秒前
3秒前
3秒前
cg完成签到 ,获得积分10
3秒前
Pyc完成签到 ,获得积分10
3秒前
四月胧完成签到,获得积分10
4秒前
华仔应助你还要猫怎样采纳,获得10
5秒前
5秒前
黄老板发布了新的文献求助10
7秒前
wt200001发布了新的文献求助10
8秒前
科研通AI2S应助TiO太阳采纳,获得10
8秒前
8秒前
9秒前
益安完成签到,获得积分10
9秒前
Andy完成签到,获得积分10
10秒前
10秒前
共享精神应助司徒无剑采纳,获得10
11秒前
11秒前
学术发布了新的文献求助10
12秒前
蛋筒发布了新的文献求助10
12秒前
seusyy发布了新的文献求助20
12秒前
13秒前
13秒前
ding应助Una采纳,获得10
14秒前
14秒前
一衣带水发布了新的文献求助10
14秒前
14秒前
小薇完成签到,获得积分20
15秒前
15秒前
15秒前
椒盐丸子发布了新的文献求助10
16秒前
16秒前
传奇3应助聂学雨采纳,获得10
16秒前
19秒前
水三寿完成签到,获得积分20
19秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3134791
求助须知:如何正确求助?哪些是违规求助? 2785712
关于积分的说明 7773726
捐赠科研通 2441524
什么是DOI,文献DOI怎么找? 1297985
科研通“疑难数据库(出版商)”最低求助积分说明 625075
版权声明 600825