癌细胞
细胞凋亡
PI3K/AKT/mTOR通路
肺癌
细胞毒性T细胞
癌症研究
巨噬细胞
化学
癌症
巨噬细胞极化
蛋白激酶B
分泌物
生物
医学
生物化学
体外
内科学
遗传学
作者
Zhi‐Hu Lin,Mei‐Kuang Lu,Hung-Chih Lo,Chia-Chuan Chang,Ai-Jung Tseng,Chi‐Hsein Chao,Tung‐Yi Lin
标识
DOI:10.1016/j.ijbiomac.2023.124144
摘要
Sulfated polysaccharides (Ac-SPSs) of Antrodia cinnamomea present anti-cancer activity. However, the anti-cancer mechanism of Ac-SPSs is not fully understood and remains largely unexplored. In this study, we identify an Ac-SPS with 7.9 kDa, noted ZnF3, and aim to examine the dual anti-cancer functions of ZnF3 on inhibiting cancer cells and activating macrophages. A biological study shows that ZnF3 inhibits lung cancer cells by inducing subG1 population and apoptosis. ZnF3 downregulates the expression of TGFβ receptor in lung cancer cells. In parallel, ZnF3 activates macrophages via induction of TNF-α and IL-6 secretion, NO production and phagocytosis. ZnF3 activates AKT/mTOR pathway and induces M1 type macrophage polarization. Cancer cells co-cultured with ZnF3-stimulated macrophages, leading to inhibition of lung cancer cells. This study demonstrates that ZnF3 not only directly inhibits cancer cells but also activates macrophages-mediated cytotoxic effect on cancer cells. Moreover, ZnF3 may be a supplement for suppressing lung cancer cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI