LPS‐Induced Mitochondrial Dysfunction Reduces Oocyte Maturation and Developmental Competence of Buffalo Embryos via ROS Mediated TLR4 Signalling

胚泡 SOD2 卵母细胞 男科 生物 体外成熟 TLR4型 活性氧 细胞生物学 胚胎发生 胚胎 内分泌学 信号转导 氧化应激 超氧化物歧化酶 医学
作者
Sujata Jinagal,Ravi Dutt,Maninder Sharma,Meeti Punetha,Sheetal Saini,Swati Thakur,Suman Chaudhary,Pradeep Kumar,Prem Singh Yadav,Tirtha Kumar Datta,Dharmendra Kumar
出处
期刊:American Journal of Reproductive Immunology [Wiley]
卷期号:92 (1)
标识
DOI:10.1111/aji.13902
摘要

ABSTRACT Problem Lipopolysaccharide (LPS) from gram‐negative bacteria has reportedly been associated with infectious diseases like metritis, which has a substantial adverse effect on animal reproductive performance and causes serious financial losses for the dairy sector. The current work aimed to establish the impact of LPS on in vitro oocyte maturation and subsequent in vitro developmental competence of oocytes, as well as to investigate the explanatory molecular mechanism underlying this effect. Method of Study Buffalo cumulus‐oocyte complexes (COCs) were challenged with 0, 5, 10 and 20 µg/mL LPS during IVM followed by IVF and IVC. Cytoplasmic and nuclear maturation, cleavage and blastocyst rate, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP, ΔΨm) and transcript abundance of genes related to inflammation, antioxidation and apoptosis were evaluated. Results The maturation and subsequent embryonic development competency were found to be significantly ( p ≤ 0.05) reduced with the addition of 10 and 20 µg/mL LPS to IVM media. ROS production accompanied by a decreased ΔΨm was recorded in LPS‐treated oocytes in comparison to the control group ( p ≤ 0.05). Our results were further supported by the transcriptional expression of proinflammatory (TLR4, CD14 and RPS27A) and apoptotic gene (Caspase 3) which were found to be significantly increased while antioxidant genes (SOD2 and GPX1) were decreased significantly in matured oocytes and blastocyst after LPS exposure. Conclusions The deleterious effects of LPS are mediated through ROS generation, which triggers inflammatory processes via the TLR4 pathway and impairs oocyte maturation and subsequent embryonic development.
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