LPS‐Induced Mitochondrial Dysfunction Reduces Oocyte Maturation and Developmental Competence of Buffalo Embryos via ROS Mediated TLR4 Signalling

胚泡 SOD2 卵母细胞 男科 生物 体外成熟 TLR4型 活性氧 细胞生物学 胚胎发生 胚胎 内分泌学 信号转导 氧化应激 超氧化物歧化酶 医学
作者
Sujata Jinagal,Ravi Dutt,Maninder Sharma,Meeti Punetha,Sheetal Saini,Swati Thakur,Suman Chaudhary,Pradeep Kumar,Prem Singh Yadav,Tirtha Kumar Datta,Dharmendra Kumar
出处
期刊:American Journal of Reproductive Immunology [Wiley]
卷期号:92 (1)
标识
DOI:10.1111/aji.13902
摘要

ABSTRACT Problem Lipopolysaccharide (LPS) from gram‐negative bacteria has reportedly been associated with infectious diseases like metritis, which has a substantial adverse effect on animal reproductive performance and causes serious financial losses for the dairy sector. The current work aimed to establish the impact of LPS on in vitro oocyte maturation and subsequent in vitro developmental competence of oocytes, as well as to investigate the explanatory molecular mechanism underlying this effect. Method of Study Buffalo cumulus‐oocyte complexes (COCs) were challenged with 0, 5, 10 and 20 µg/mL LPS during IVM followed by IVF and IVC. Cytoplasmic and nuclear maturation, cleavage and blastocyst rate, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP, ΔΨm) and transcript abundance of genes related to inflammation, antioxidation and apoptosis were evaluated. Results The maturation and subsequent embryonic development competency were found to be significantly ( p ≤ 0.05) reduced with the addition of 10 and 20 µg/mL LPS to IVM media. ROS production accompanied by a decreased ΔΨm was recorded in LPS‐treated oocytes in comparison to the control group ( p ≤ 0.05). Our results were further supported by the transcriptional expression of proinflammatory (TLR4, CD14 and RPS27A) and apoptotic gene (Caspase 3) which were found to be significantly increased while antioxidant genes (SOD2 and GPX1) were decreased significantly in matured oocytes and blastocyst after LPS exposure. Conclusions The deleterious effects of LPS are mediated through ROS generation, which triggers inflammatory processes via the TLR4 pathway and impairs oocyte maturation and subsequent embryonic development.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
fang应助科研通管家采纳,获得10
刚刚
共享精神应助科研通管家采纳,获得10
刚刚
山野村夫应助科研通管家采纳,获得10
1秒前
zz应助科研通管家采纳,获得10
1秒前
Singularity应助科研通管家采纳,获得10
1秒前
Singularity应助科研通管家采纳,获得10
1秒前
1秒前
1秒前
1秒前
赘婿应助nkmenghan采纳,获得10
1秒前
芝麻福福完成签到,获得积分10
1秒前
快乐枫发布了新的文献求助10
1秒前
blue发布了新的文献求助10
2秒前
晚灯君完成签到 ,获得积分10
3秒前
UU发布了新的文献求助10
3秒前
lrll完成签到,获得积分10
5秒前
5秒前
lele完成签到,获得积分10
5秒前
hhh发布了新的文献求助10
8秒前
我要看文献完成签到 ,获得积分10
8秒前
wdd完成签到 ,获得积分10
10秒前
李爱国应助开心薯片采纳,获得10
11秒前
12秒前
Z_yiming发布了新的文献求助10
12秒前
爱吃草莓和菠萝的吕可爱完成签到,获得积分10
14秒前
缥缈一刀发布了新的文献求助10
16秒前
小地蛋完成签到 ,获得积分10
17秒前
感谢大哥的帮助完成签到 ,获得积分10
17秒前
614521完成签到,获得积分10
19秒前
111完成签到,获得积分10
20秒前
王先生完成签到 ,获得积分10
20秒前
21秒前
殊荣完成签到,获得积分10
22秒前
JAMA兜里揣完成签到,获得积分10
22秒前
lalala完成签到 ,获得积分10
24秒前
王三歲完成签到,获得积分10
25秒前
你的背包完成签到,获得积分10
25秒前
Yang应助快乐枫采纳,获得10
26秒前
冷傲的如柏完成签到,获得积分10
27秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038303
求助须知:如何正确求助?哪些是违规求助? 3576013
关于积分的说明 11374210
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029