荧光
氨基酸
荧光蛋白
化学
生物化学
绿色荧光蛋白
基因
量子力学
物理
作者
Aysilu N. Asanova,Oksana M. Subach,Sofya A. Myachina,Marta Evteeva,Natalia M. Gunitseva,Anna A. Borisova,М. В. Патрушев,Anna V. Vlaskina,A.Y. Nikolaeva,Lina Yang,Azat Gabdulkhakov,Elizaveta A. Dronova,В. Р. Самыгина,Xian Xiao,Hu Zhao,Kiryl D. Piatkevich,Fedor V. Subach
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2024-10-14
卷期号:9 (10): 5135-5147
标识
DOI:10.1021/acssensors.4c01055
摘要
Branched-chain amino acids (BCAAs) play an important role in the functioning of mammalian cells and the central nervous system. However, available genetically encoded indicators for BCAAs are based on Förster resonance energy transfer and have a limited dynamic range. We developed a single fluorescent protein-based sensor for BCAAs, called NeIle, which is composed of circularly permutated mNeonGreen protein inserted into the leucine-isoleucine-valine binding protein (LIVBP) from
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