适体
检出限
化学发光
色谱法
化学
冈田酸
辣根过氧化物酶
微流控
鲁米诺
纳米技术
材料科学
生物化学
分子生物学
酶
磷酸酶
生物
磷酸化
作者
Libing Mao,Qi Zhao,Yan Yang,Qianqian Wang,Yiyang Dong
出处
期刊:Separations
[MDPI AG]
日期:2022-11-07
卷期号:9 (11): 350-350
被引量:2
标识
DOI:10.3390/separations9110350
摘要
Rapid detection of okadaic acid (OA) in shellfish is crucial for practical application in food safety analysis. In order to establish a rapid, delicate detection scheme, an OA aptamer was utilized to quickly capture OA from the sample solution with polystyrene microspheres as solid phase carriers, and an inner-microchannel dam structure was designed to intercept the aptamer-functionalized microspheres to achieve the separation of OA for detection. Horseradish peroxidase (HRP) is utilized to catalyze the luminescence reaction of luminol-H2O2 solution. Through the direct competition for the aptamer between OA and OA-HRP, the rapid detection of OA can be achieved. The dynamic range of this detection method is 41.3–4.02 ng/mL, and the limit of detection (LOD) and lowest limit of quantitation (LOQ) are 12.4 pg/mL and 41.3 pg/mL, respectively. This miniaturized device enables rapid, ultrasensitive detection of OA, and demonstrates the merits of its field portability and low reagent consumption. The device can be deployed for on-site detection and analysis of marine biotoxins thereof.
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