细胞毒性T细胞
阳离子脂质体
CD8型
体外
癌症研究
体内
免疫学
细胞
细胞培养
生物
化学
免疫系统
转染
生物化学
遗传学
生物技术
作者
Rosalva Rangel-Corona,Teresa Corona-Ortega,Itzel del Río-Ortiz,Miriam Nieves,Hirán Morán-Bañuelos,Olivia González-Tenorio,Julio Roberto Cáceres‐Cortés,Benny Weiss‐Steider
标识
DOI:10.3109/10611861003733920
摘要
Tumor cells are known to modify their surroundings in order to escape immunologic detection, and IL-2, a killer cell activator, is one of the factors known to overcome this escape mechanism. In this regard, when we cocultured cells from the human cervical cancer cell line INBL with mice blood leukocytes, no inhibition of tumor cell growth was observed, but when a similar coculture was done in the presence of cationic liposomes bearing IL-2 on their external surface (CL-IL-2), all the INBL cells were killed. In order to evaluate whether this in vitro property of CL-IL-2 to overcome tumor cell detection by lymphocytes could also be reproduced in vivo, INBL cells were intraperitoneally (i.p.) inoculated into immunodepressed mice to produce solid tumors. We observed that the subsequent i.p. delivery of CL-IL-2 rendered the tumor masses significantly smaller. The presence of a large number of infiltrating lymphocytes on those tumors, and the fact that many had a cytotoxic CD8+ phenotype suggests that these lymphocytes were responsible for the observed antitumor effect. Finally, the possible formation of a bridge between the IL-2R receptors on both, the lymphocytes and the INBL cells, mediated by the IL-2-bearing liposomes, and its possible effect on the activation of antitumor cytotoxic lymphocytes is discussed.
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