昼夜节律
表达式(计算机科学)
计算生物学
生物
计算机科学
神经科学
程序设计语言
作者
Shin Yamazaki,Joseph S. Takahashi
出处
期刊:Methods in Enzymology
日期:2005-01-01
卷期号:: 288-301
被引量:183
标识
DOI:10.1016/s0076-6879(05)93012-7
摘要
Luminescence reporters have been used successfully in studies of circadian rhythms. Real-time measurements of circadian variations in gene expression were made in living cells, cultured tissues, and whole organisms. Because this technique is relatively easy and continuous noninvasive measurement from tissue cultures allows for a drastic reduction in the number of experimental animals, we believe this method will become a common technique for studying circadian rhythms. Using a multichannel recording apparatus, it may also become a powerful tool for the discovery of new drugs. In the past, measurements were done using hand-made apparatuses or by modifying commercially available equipment. We, along with other investigators, have developed user-friendly equipment for performing circadian rhythms experiments, and these systems are now available commercially. This article describes the use of luminescence reporters in circadian research and provides detailed methods used in these experiments. One of our goals in this article is to reduce experimental variability in different laboratories by proposing standard protocols.
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