Cloning and expression analysis of glucose transporter 4 mRNA in the cold hardiness frog, Rana dybowskii.

The Rana dybowskii distribute in northeast region of China which have seasonally cold climates. During winter they survival freezing by biosynthesizing carbohydrate cryoprotectants such as high concentrations glucose into blood and all tissues. The essential role of glucose transporter 4 is a high-affinity glucose transporter, which can increase glucose uptake in cells when it stimulated by insulin.In this study, we analysis the full-length GLUT4 mRNA detect the gene levels of GLUT4 in R. dybowskii main tissues by qPCR during low temperature.We found in heart, fat body, skeletal muscle and skin four tissues all express GLUT4, and the levels of GLUT4 decreased on initial cold exposure stage, 8~12 hours, followed 24 hours it recovered.This study we firstly indentified and characterized GLUT4 in amphibious, and provide a novel insight into the role of GLUT4 in cryoprotectant synthesis and cell protection in cold hardiness amphibians.