生物相容性
溶血
牛血清白蛋白
材料科学
检出限
荧光
水溶液
纳米技术
化学
人血清白蛋白
生物传感器
化学工程
核化学
色谱法
有机化学
工程类
物理
生物
免疫学
量子力学
作者
Manisha Kumari,Savita Chaudhary
标识
DOI:10.1016/j.colsurfb.2020.111333
摘要
The reliable means for the quick recognition of pathogenic bacteria for disease prevention has acquired a considerable significance among researchers for human wellbeing. The purpose of current study is to fabricate highly photo-luminescent and biocompatible carbon dots (CQDs) from single used plastic waste such as poly bags, cups and bottles for selective sensing of E. coli. The formed small sized spherical CQDs have possessed high water solubility with excellent photo stable nature. The detailed fluorescence emission studies of CQDs have illustrated the overview of different types of surface defects and emissive states in formed particles. In addition, the specific role of prepared CQDs with bio-compatible nature has been thoroughly checked on hemolysis of red blood cells by employing in-vitro blood compatibility assay. A combination of anticoagulant and thrombolytic activity of CQDs with anti-oxidative activities from reactive oxygen species (ROS) generation were found to be responsible for the biocompatibility of formed CQDs. The effective in vitro stability toward histidine, cysteine, bovine serum albumin (BSA), human serum albumin (HSA) and saline supported the biocompatibility of formed particles as a function of different concentrations of CQDs. The optimized fluorescence properties with high surface to volume ratio of CQDs have provided a better alternative for “turn-off” fluorescence detection of E. coil in aqueous media with limit of detection down to 108 CFU/mL estimated by using standard colony-counting method. The as prepared nanosensor provided easy fabrication with fast response, high sensitivity and selectivity in real water samples which might be necessary for detecting pathogenic agents. In addition, the current work has provided the large overview in blood compatibility and anti-oxidative potential of prepared CQDs and giving useful information for preparing blood–compatible nanoparticles with enhanced bacterial discrimination ability.
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