Development of Infectious Clone of Virus

An infectious clone is a full-length DNA clone from which infectious transcripts can be obtained in vitro or in vivo with a suitable promoter. An infectious clone is the basic need to study functional genomics, replication and expression of viral proteins and in understanding host–virus interactions. Infectious clones, considered as the pools of viral genes for designing antiviral strategies, are an essential source of material for the preparation of new viral vectors. In this chapter, production of in vitro and in vivo clones of viruses is discussed.