纳米孔
纳米技术
生物分子
微流控
表面改性
生物传感器
材料科学
聚合物
纳米颗粒
膜
分析物
化学
色谱法
生物化学
物理化学
复合材料
作者
Izadora Mayumi Fujinami Tanimoto,Benjamin Cressiot,Nathalie Jarroux,Jean Roman,G. Patriarche,Bruno Le Pioufle,Juan Pelta,Laurent Bacri
标识
DOI:10.1016/j.bios.2021.113195
摘要
Solid-state nanopores provide a powerful tool to electrically analyze nanoparticles and biomolecules at single-molecule resolution. These biosensors need to have a controlled surface to provide information about the analyte. Specific detection remains limited due to nonspecific interactions between the molecules and the nanopore. Here, a polymer surface modification to passivate the membrane is performed. This functionalization improves nanopore stability and ionic conduction. Moreover, one can control the nanopore diameter and the specific interactions between protein and pore surface. The effect of ionic strength and pH are probed. Which enables control of the electroosmotic driving force and dynamics. Furthermore, a study of polymer chain structure and permeability in the pore are carried out. The nanopore chip is integrated into a microfluidic device to ease its handling. Finally, a discussion of an ionic conductance model through a permeable crown along the nanopore surface is elucidated. The proof of concept is demonstrated by the capture of free streptavidin by the biotins grafted into the nanopore. In the future, this approach could be used for virus diagnostic, nanoparticle or biomarker sensing.
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