微泡
外体
流式细胞术
生物标志物
微流控
纳米技术
检出限
化学
计算生物学
生物
材料科学
分子生物学
色谱法
生物化学
小RNA
基因
作者
Xinzhuo Liu,Zhiyou Zong,Mindan Xing,Xuehui Liu,Jia Li,Dingbin Liu
出处
期刊:Nano Letters
[American Chemical Society]
日期:2021-10-05
卷期号:21 (20): 8817-8823
被引量:37
标识
DOI:10.1021/acs.nanolett.1c03211
摘要
Exosomes have recently emerged as some of the most promising biomarkers for disease diagnosis. Due to their small sizes and composition heterogeneity, exosomes are difficult to detect by currently available platforms. Here, we report a pH-mediated assembly system that converts single nanosized exosomes into microsized clusters, which can be directly analyzed by conventional flow cytometry (FCM), breaking through the size limit of exosome analysis. We demonstrated the clinical utility of the pH-mediated clustering system by profiling the exosomal proteins from blood plasma samples of 33 cancer patients and 11 benign controls. The results indicated that the combination of MUC-1 and PD-L1 could serve as a new biomarker panel for the early diagnosis of liver cancer with high clinical accuracy. This pH-mediated assembly strategy allows rapid, sensitive, and high-throughput analysis of exosome biomarkers by conventional FCM, which can be easily refined for use in both basic and clinical settings.
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