蛋白酶
水解
酶
碱性蛋白酶
酶水解
化学
生物化学
固定化酶
中性蛋白酶
色谱法
作者
Wilhelm R. Glomm,Sileshi Gizachew Wubshet,Diana Lindberg,Katinka R. Dankel,Nils Kristian Afseth,Per Stenstad,Heidi Johnsen
标识
DOI:10.1016/j.lwt.2021.112327
摘要
Immobilization of enzymes onto magnetic particles can potentially allow for enzyme reuse, which can significantly reduce the cost associated with e.g. enzymatic protein hydrolysis (EPH) of fish and meat by-products. Here, we report glutaraldehyde-mediated immobilization of a food-grade protease (Subtilisin A) onto magnetic silica particles using three different amine ligands; a short, brush-like linker (aminopropyl trimethoxysilane), a long, flexible linker (Jeffamine), and a gel-like coating (chitosan). The three coupling strategies were evaluated and compared with respect to the amount of immobilized protease, enzyme activity and catalytic performance in the hydrolysis of chicken meat and turkey tendons, respectively. The particle systems showed high reusability (≤85% activity remaining after six consecutive cycles) and storage stability (≤93% activity remaining after 25 months storage). Particle-immobilized enzyme systems were able to catalyze degradation and extraction of protein from chicken meat and turkey tendons. • Subtilisin A can be immobilized onto magnetic particles with different coatings. • Immobilized Subtilisin A can degrade protein from chicken meat and turkey tendons. • Subtilisin A coupled to magnetic particles is highly reusable and storage stable.
科研通智能强力驱动
Strongly Powered by AbleSci AI