Normal concentration range of blood mercury and bone mineral density: a cross-sectional study of National Health and Nutrition Examination Survey (NHANES) 2005–2010

High blood mercury levels could lead to mercury poisoning, undoubtedly causing great harm to human health. However, the impact of the normal concentration of blood mercury on bone mineral density (BMD) is unclear. Therefore, this study explored the relationship between blood mercury levels and BMD and determined whether the relationship between blood mercury and BMD differs by populations. Two researchers extracted data from the 2005-2010 National Health and Nutrition Examination Survey database. Multivariate linear regression models were performed to evaluate the relationship between mercury level and BMD of the femoral regions and spine. Subgroup analysis was used to estimate differences according to population subgroups. Moreover, the nonlinear relationship of blood mercury levels and BMD was assessed using smooth curve fitting and generalized additive models. The results showed increased BMD with increasing mercury levels by multivariable-adjusted linear regression models, especially in the femoral regions. Subgroup analysis showed that the relationship was more likely to be present in non-Hispanic Whites, while a negative correlation between blood mercury levels and spinal BMD was observed in non-Hispanic Blacks. Furthermore, males (aged 20 to 29 years) and females (aged 30 to 39 years) with low blood mercury levels (< 3 ug/L) had increased risks of osteopenia or osteoporosis. This study showed that blood mercury level within the normal reference value of 10 μg/dL may be associated with BMD, especially with a lower blood mercury level, which may suggest an elevated risk of osteopenia or osteoporosis. However, causation could not be established due to the study design.