生物相容性
细胞毒性
金属有机骨架
咪唑酯
药物输送
体内
纳米技术
材料科学
化学
生物物理学
体外
生物化学
有机化学
生物技术
生物
吸附
作者
Marcus Hoop,Claudio F. Walde,Raffaele Riccò,Fajer Mushtaq,Anastasia Terzopoulou,Xiang‐Zhong Chen,Andrew J. deMello,Christian J. Doonan,Paolo Falcaro,Bradley J. Nelson,Josep Puigmartí‐Luis,Salvador Pané
标识
DOI:10.1016/j.apmt.2017.12.014
摘要
Metal–organic frameworks (MOFs) are a class of crystalline materials constructed from organic linkers and inorganic nodes. MOFs typically possess ultra-high surface areas and pore volumes; thus, they are ideal candidates for biomedical applications. Zinc Imidazolate Framework 8 (ZIF-8) has been widely established in the literature as a potential candidate for on-demand drug delivery applications. Indeed, ZIF-8 has a remarkable loading capacity, stability in physiological environments, and tunable drug release properties. However, the use of ZIF-8 for in vivo applications requires a clear understanding of the interaction of ZIF-8 with biological tissue. In this work, we investigated the biocompatibility of ZIF-8 toward six different cell lines representing various body parts (kidney, skin, breast, blood, bones, and connective tissue). Our results suggest that ZIF-8 has no significant cytotoxicity up to a threshold value of 30 μg mL−1. Above 30 μg mL−1, the cytotoxicity is shown to result from the influence of released Zinc ions (Zn2+) on the mitochondrial ROS production. This adverse effect is responsible for cell cycle arrest in the G2/M phase due to irreversible DNA damage, ultimately initiating cellular apoptosis pathways. Due to this insight, we encapsulated a hormone, insulin, into ZIF-8 particles and then compared its drug delivery capabilities to the aforementioned cytotoxicity values. Our results suggest that ZIF-8 is suitable for therapeutic applications. Furthermore, this study establishes a clear understanding of the interaction of ZIF-8 and its constituents with various cell lines and highlights the important biocompatibility factors that must be considered for future in vivo testing.
科研通智能强力驱动
Strongly Powered by AbleSci AI