Effects of 3-butenyl isothiocyanate on phenotypically different prostate cancer cells

DU145型 细胞毒性T细胞 台盼蓝 多西紫杉醇 细胞凋亡 异硫氰酸盐 前列腺癌 细胞培养 癌细胞 癌症研究 癌症 生物 体外 癌基因 顺铂 药理学 LNCaP公司 细胞周期 内科学 医学 生物化学 化疗 遗传学
作者
M.j. N��ez-Iglesias,Silvia Novío,C. Garc�a-Santiago,María Elena Cartea,Pilar Soengas,Pablo Velasco,Manuel Freire‐Garabal
出处
期刊:International Journal of Oncology [Spandidos Publications]
被引量:13
标识
DOI:10.3892/ijo.2018.4545
摘要

Isothiocyanates (ITCs) have gained increasing attention since they have been attributed the merits for the potential beneficial effects of cruciferous vegetable dietary consumption on cancer. The aim of the present study was to determine the cytotoxic effects of 3-butenyl ITC (3-BI) on prostate cancer (PC) cells under in vitro conditions. Two androgen-insensitive human PC cell lines, PC-3 and DU145, were assayed. Cells were cultured in the presence of increasing concentrations of 3-BI (5, 10, 30 and 50 µM) in the absence or presence of the chemotherapeutic drug docetaxel (DOCE) (1 and 2 nM). The cytotoxic effects of these compounds were analyzed using the trypan blue exclusion assay at 24, 48 and 72 h. Apoptosis and migration assays were also performed. The results showed that 3-BI induced a dose-dependent cytotoxic effect on PC-3 cells at 24, 48 and 72 h. These effects were significantly higher than those found with DOCE at 72 h of culture. Moreover, 3-BI also potentiated the effects of DOCE in a dose-dependent manner. Additionally, 3-BI showed inhibition of the migration of PC-3 cells. Nevertheless, 3-BI was not effective in the DU145 PC cell line. These results show a promising role for the 3-BI compound as a co-adjuvant agent in DOCE-based therapy in certain types of PC.

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