藻胆蛋白
藻蓝蛋白
联合囊肿
别藻蓝蛋白
化学
食品科学
蓝藻
类囊体
生物化学
生物
叶绿体
细菌
遗传学
基因
突变体
作者
Zahra Zahra,Seok-Young Kim,Hye-Youn Kim,Hwanhui Lee,Heayyean Lee,Jun-Yeong Jeon,Dong‐Min Kim,Dong‐Myung Kim,Seong-Joo Hong,Byung‐Kwan Cho,Hookeun Lee,Choul-Gyun Lee,Muhammad Arshad,Hyung‐Kyoon Choi
标识
DOI:10.1021/acs.jafc.8b01522
摘要
This study aimed to improve the production of phycobiliproteins using TiO2 nanoparticles (NPs) in Synechocystis sp. PCC 6803. The growth characteristics of Synechocystis cells were not affected by TiO2 NPs treatment, but this treatment increased the chlorophyll content significantly by 62.2% (14.6 mg/L) compared to that of control (9.0 mg/L) on day 16. Phycocyanin production was increased by 33.8% (29.3 g/L) compared to that of control (21.9 g/L) on day 8. Allophycocyanin production was increased by 55.0% (6.2 g/L) compared to that of control (4.0 g/L) on day 8, and by 22.4% (16.4 g/L) compared to that of control (13.4 g/L) on day 16. Direct infusion mass spectrometry revealed that TiO2 NPs treatment significantly increased the levels of major thylakoid membranes of monogalactosyldiacylglycerols (18:2/18:3, 18:2/18:2, 18:1/18:2), phosphatidylglycerol (16:0/16:1), and sulfoquinovosyldiacylglycerols (16:0/16:1, 16:0:18:4) on day 8. These findings indicate that TiO2 NPs have potential for commercial applications in Synechocystis species or other microalgal strains.
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