类有机物
免疫染色
导管细胞
细胞生物学
生物
祖细胞
细胞培养
基质凝胶
体内
细胞
细胞外基质
干细胞
病理
胰腺
免疫学
医学
免疫组织化学
生物技术
生物化学
遗传学
作者
Habib Rezanejad,J Lock,Benjamin D. Sullivan,Susan Bonner-Weir
摘要
Abstract Traditionally, studies of cells and tissues have been performed on isolated primary cells or immortalized cell lines by culturing them in 2D culture dishes or flasks. However, a caveat regarding 2D culture is that the cells poorly recapitulate their in vivo counterparts, mainly due to a lack of 3D cell‐cell and cell–extracellular matrix interactions. In recent years, the development of in vitro organoids as 3D culture has gained substantial attention as a model to study different tissues. In adults, pancreatic ductal cells are considered as a source of stem or progenitor cells, so developing new methods to study ductal cells would be useful. Here, we provide a protocol to isolate mouse pancreatic ductal cells and a cost‐effective protocol to generate 3D organoid structures from such ductal cells. Additionally, we have devised a protocol for immunostaining of intact whole organoids without sectioning. © 2018 by John Wiley & Sons, Inc.
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