Advax augments B and T cell responses upon influenza vaccination via the respiratory tract and enables complete protection of mice against lethal influenza virus challenge

佐剂 免疫学 免疫系统 免疫 接种疫苗 呼吸道 鼻腔给药 流感疫苗 病毒学 病毒 医学 呼吸系统 生物 内科学
作者
Jasmine Tomar,Harshad P. Patil,Gustavo Bracho,Wouter F. Tonnis,Henderik W. Frijlink,Nikolai Petrovsky,Rita Vanbever,Anke Huckriede,Wouter L.J. Hinrichs
出处
期刊:Journal of Controlled Release [Elsevier]
卷期号:288: 199-211 被引量:46
标识
DOI:10.1016/j.jconrel.2018.09.006
摘要

Administration of influenza vaccines via the respiratory tract has potential benefits over conventional parenteral administration, inducing immunity directly at the site of influenza exposure as well as being needle free. In this study, we investigated the suitability of Advax™, a stable particulate polymorph of inulin, also referred to as delta inulin, as a mucosal adjuvant for whole inactivated influenza vaccine (WIV) administered either as a liquid or dry powder formulation. Spray freeze-drying produced Advax-adjuvanted WIV powder particles in a size range (1-5 μm) suitable for inhalation. The physical and biological characteristics of both WIV and Advax remained unaltered both by admixing WIV with Advax and by spray freeze drying. Upon intranasal or pulmonary immunization, both liquid and dry powder formulations containing Advax induced significantly higher systemic, mucosal and cellular immune responses than non-adjuvanted WIV formulations. Furthermore, pulmonary immunization with Advax-adjuvanted WIV led to robust memory B cell responses along with an increase of lung localization factors i.e. CXCR3, CD69, and CD103. A less pronounced but still positive effect of Advax was seen on memory T cell responses. In contrast to animals immunized with WIV alone, all animals pulmonary immunized with a single dose of Advax-adjuvanted WIV were fully protected with no visible clinical symptoms against a lethal dose of influenza virus. These data confirm that Advax is a potent mucosal adjuvant that boosts vaccine-induced humoral and cellular immune responses both in the lung and systemically with major positive effects on B-cell memory and complete protection against live virus. Hence, respiratory tract immunization, particularly via the lungs, with Advax-adjuvanted WIV formulation as a liquid or dry powder is a promising alternative to parenteral influenza vaccination.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
leeteukxx完成签到,获得积分10
3秒前
李昀睿完成签到,获得积分20
4秒前
4秒前
4秒前
小一发布了新的文献求助10
5秒前
5秒前
李昀睿发布了新的文献求助10
8秒前
8秒前
执着月饼发布了新的文献求助20
9秒前
骑着蜗牛去赶集完成签到,获得积分10
10秒前
zz发布了新的文献求助10
10秒前
Tiamo发布了新的文献求助10
10秒前
10秒前
甜蜜的白梦完成签到,获得积分10
10秒前
10秒前
11秒前
11秒前
ding应助YUMMY采纳,获得10
11秒前
廾匸发布了新的文献求助10
12秒前
无意的寻琴应助顺心绮兰采纳,获得10
12秒前
12秒前
butterfly发布了新的文献求助10
14秒前
14秒前
14秒前
15秒前
科研通AI2S应助科研通管家采纳,获得10
16秒前
TrucCSC应助科研通管家采纳,获得10
16秒前
宇心应助科研通管家采纳,获得10
16秒前
英俊的铭应助科研通管家采纳,获得10
16秒前
赘婿应助科研通管家采纳,获得30
16秒前
李健应助李昀睿采纳,获得10
16秒前
CodeCraft应助科研通管家采纳,获得10
16秒前
SciGPT应助科研通管家采纳,获得10
16秒前
英俊的铭应助科研通管家采纳,获得10
16秒前
Singularity应助科研通管家采纳,获得10
17秒前
充电宝应助科研通管家采纳,获得10
17秒前
打打应助科研通管家采纳,获得10
17秒前
乐乐应助科研通管家采纳,获得30
17秒前
NexusExplorer应助科研通管家采纳,获得10
17秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3182487
求助须知:如何正确求助?哪些是违规求助? 2832839
关于积分的说明 7990824
捐赠科研通 2494830
什么是DOI,文献DOI怎么找? 1331053
科研通“疑难数据库(出版商)”最低求助积分说明 636228
邀请新用户注册赠送积分活动 603191