亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Advax augments B and T cell responses upon influenza vaccination via the respiratory tract and enables complete protection of mice against lethal influenza virus challenge

佐剂 免疫学 免疫系统 免疫 接种疫苗 呼吸道 鼻腔给药 流感疫苗 病毒学 病毒 医学 呼吸系统 生物 内科学
作者
Jasmine Tomar,Harshad P. Patil,Gustavo Bracho,Wouter F. Tonnis,Henderik W. Frijlink,Nikolai Petrovsky,Rita Vanbever,Anke Huckriede,Wouter L.J. Hinrichs
出处
期刊:Journal of Controlled Release [Elsevier]
卷期号:288: 199-211 被引量:46
标识
DOI:10.1016/j.jconrel.2018.09.006
摘要

Administration of influenza vaccines via the respiratory tract has potential benefits over conventional parenteral administration, inducing immunity directly at the site of influenza exposure as well as being needle free. In this study, we investigated the suitability of Advax™, a stable particulate polymorph of inulin, also referred to as delta inulin, as a mucosal adjuvant for whole inactivated influenza vaccine (WIV) administered either as a liquid or dry powder formulation. Spray freeze-drying produced Advax-adjuvanted WIV powder particles in a size range (1-5 μm) suitable for inhalation. The physical and biological characteristics of both WIV and Advax remained unaltered both by admixing WIV with Advax and by spray freeze drying. Upon intranasal or pulmonary immunization, both liquid and dry powder formulations containing Advax induced significantly higher systemic, mucosal and cellular immune responses than non-adjuvanted WIV formulations. Furthermore, pulmonary immunization with Advax-adjuvanted WIV led to robust memory B cell responses along with an increase of lung localization factors i.e. CXCR3, CD69, and CD103. A less pronounced but still positive effect of Advax was seen on memory T cell responses. In contrast to animals immunized with WIV alone, all animals pulmonary immunized with a single dose of Advax-adjuvanted WIV were fully protected with no visible clinical symptoms against a lethal dose of influenza virus. These data confirm that Advax is a potent mucosal adjuvant that boosts vaccine-induced humoral and cellular immune responses both in the lung and systemically with major positive effects on B-cell memory and complete protection against live virus. Hence, respiratory tract immunization, particularly via the lungs, with Advax-adjuvanted WIV formulation as a liquid or dry powder is a promising alternative to parenteral influenza vaccination.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
明理友容完成签到 ,获得积分10
1秒前
冷静的小之完成签到,获得积分10
5秒前
wanci应助DOCTORLI采纳,获得10
7秒前
。。。完成签到,获得积分10
9秒前
9秒前
淡淡妙竹完成签到 ,获得积分10
16秒前
xxxx完成签到 ,获得积分10
19秒前
26秒前
30秒前
33秒前
顾矜应助林厌寻采纳,获得10
33秒前
华仔应助小白采纳,获得10
38秒前
40秒前
排骨炖豆角完成签到 ,获得积分10
40秒前
甜美的问蕊完成签到 ,获得积分10
40秒前
41秒前
42秒前
小王日记完成签到,获得积分10
42秒前
栗悟饭完成签到,获得积分10
43秒前
46秒前
林厌寻发布了新的文献求助10
46秒前
46秒前
46秒前
DOCTORLI发布了新的文献求助10
48秒前
北笙发布了新的文献求助10
50秒前
zac完成签到 ,获得积分10
53秒前
草莓奶昔完成签到 ,获得积分10
58秒前
Orange应助黑小羿采纳,获得30
1分钟前
dxwy完成签到,获得积分10
1分钟前
大模型应助兴奋的宛亦采纳,获得10
1分钟前
1分钟前
儒雅的若翠完成签到,获得积分10
1分钟前
CodeCraft应助科研通管家采纳,获得10
1分钟前
天天快乐应助科研通管家采纳,获得10
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
杳鸢应助科研通管家采纳,获得20
1分钟前
兴奋的宛亦完成签到,获得积分20
1分钟前
卢健辉完成签到,获得积分10
1分钟前
1分钟前
海鲜汤完成签到 ,获得积分10
1分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
安全防范技术与工程 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Актуализированная стратиграфическая схема триасовых отложений Прикаспийского региона. Объяснительная записка 360
Dictionary of socialism 350
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3192491
求助须知:如何正确求助?哪些是违规求助? 2841611
关于积分的说明 8034138
捐赠科研通 2505246
什么是DOI,文献DOI怎么找? 1338462
科研通“疑难数据库(出版商)”最低求助积分说明 638351
邀请新用户注册赠送积分活动 606881