Advax augments B and T cell responses upon influenza vaccination via the respiratory tract and enables complete protection of mice against lethal influenza virus challenge

佐剂 免疫学 免疫系统 免疫 接种疫苗 呼吸道 鼻腔给药 流感疫苗 病毒学 病毒 医学 呼吸系统 生物 内科学
作者
Jasmine Tomar,Harshad P. Patil,Gustavo Bracho,Wouter F. Tonnis,Henderik W. Frijlink,Nikolai Petrovsky,Rita Vanbever,Anke Huckriede,Wouter L.J. Hinrichs
出处
期刊:Journal of Controlled Release [Elsevier]
卷期号:288: 199-211 被引量:46
标识
DOI:10.1016/j.jconrel.2018.09.006
摘要

Administration of influenza vaccines via the respiratory tract has potential benefits over conventional parenteral administration, inducing immunity directly at the site of influenza exposure as well as being needle free. In this study, we investigated the suitability of Advax™, a stable particulate polymorph of inulin, also referred to as delta inulin, as a mucosal adjuvant for whole inactivated influenza vaccine (WIV) administered either as a liquid or dry powder formulation. Spray freeze-drying produced Advax-adjuvanted WIV powder particles in a size range (1-5 μm) suitable for inhalation. The physical and biological characteristics of both WIV and Advax remained unaltered both by admixing WIV with Advax and by spray freeze drying. Upon intranasal or pulmonary immunization, both liquid and dry powder formulations containing Advax induced significantly higher systemic, mucosal and cellular immune responses than non-adjuvanted WIV formulations. Furthermore, pulmonary immunization with Advax-adjuvanted WIV led to robust memory B cell responses along with an increase of lung localization factors i.e. CXCR3, CD69, and CD103. A less pronounced but still positive effect of Advax was seen on memory T cell responses. In contrast to animals immunized with WIV alone, all animals pulmonary immunized with a single dose of Advax-adjuvanted WIV were fully protected with no visible clinical symptoms against a lethal dose of influenza virus. These data confirm that Advax is a potent mucosal adjuvant that boosts vaccine-induced humoral and cellular immune responses both in the lung and systemically with major positive effects on B-cell memory and complete protection against live virus. Hence, respiratory tract immunization, particularly via the lungs, with Advax-adjuvanted WIV formulation as a liquid or dry powder is a promising alternative to parenteral influenza vaccination.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
自觉的万言完成签到 ,获得积分10
1秒前
2秒前
2秒前
Ava应助温柔寄文采纳,获得10
2秒前
Dr大壮完成签到,获得积分10
2秒前
3秒前
稳重的八宝粥完成签到,获得积分10
5秒前
Dr大壮发布了新的文献求助10
5秒前
ZIS完成签到,获得积分10
6秒前
汉堡包应助ai化学采纳,获得10
7秒前
8秒前
8秒前
小鑫发布了新的文献求助10
10秒前
pear发布了新的文献求助10
11秒前
11秒前
11秒前
小草发布了新的文献求助10
13秒前
ssffzb2008完成签到,获得积分10
14秒前
lxy完成签到,获得积分10
15秒前
赵聚星完成签到,获得积分10
15秒前
背后卿完成签到 ,获得积分10
15秒前
李爱国应助pear采纳,获得10
18秒前
18秒前
飞飞猪发布了新的文献求助10
19秒前
赵聚星发布了新的文献求助30
20秒前
畅快的胡萝卜完成签到,获得积分10
21秒前
笨笨完成签到,获得积分10
22秒前
打工人完成签到,获得积分10
23秒前
23秒前
24秒前
24秒前
25秒前
27秒前
zhao完成签到,获得积分10
28秒前
fsz发布了新的文献求助10
30秒前
31秒前
阳光问安发布了新的文献求助10
35秒前
深情安青应助可乐采纳,获得10
36秒前
汉堡包应助fsz采纳,获得10
36秒前
39秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Dictionary of socialism 350
Mixed-anion Compounds 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3197862
求助须知:如何正确求助?哪些是违规求助? 2846631
关于积分的说明 8060019
捐赠科研通 2511518
什么是DOI,文献DOI怎么找? 1343436
科研通“疑难数据库(出版商)”最低求助积分说明 639537
邀请新用户注册赠送积分活动 609146