DPPH
化学
水解
抗氧化剂
盐酸
多糖
溶解度
核化学
酸水解
色谱法
有机化学
降级(电信)
计算机科学
电信
作者
Feng Xiong,Xia Li,Lihua Zheng,Nan Hu,Mengjia Cui,Haiyun Li
标识
DOI:10.1016/j.carbpol.2019.04.069
摘要
Water extracted polysaccharide from P. edulis peel (WPEP) was degraded using hydrochloric acid, ultrasonic-assisted hydrochloric acid, and α-amylase to obtained A-WPEP (acid hydrolysis WPEP), U-WPEP (ultrasonic-assisted acid hydrolysis WPEP), and M-WPEP (Enzymatic hydrolysis WPEP), respectively, and its relationship between structure and antioxidant activities was studied. Results showed that the solubility, internal crystalline, triple helical and surface structure of WPEP's degradation products was damaged to varying degree. Moreover, their antioxidant activities were lower compared with that of WPEP. The solubility of WPEP, A-WPEP, U-WPEP, and M-WPEP was 9.84, 6.78, 6.98, and 9.56 mg/mL, respectively. The IC50 values of their DPPH radical scavenging rates were 0.29, 1.34, 0.42 and 0.98 mg/mL, respectively. The complex formed by the degradation product with Congo red also showed different degrees of red shift in the alkaline solution. Enzymatic method had the least damage to the triple helical structure of WPEP compared with acid and ultrasonic-assisted acid method, and the better integrity of triple helical structure, the higher its antioxidant activities.
科研通智能强力驱动
Strongly Powered by AbleSci AI