代谢组学
计算生物学
生物
选择性反应监测
色谱法
质谱法
化学
串联质谱法
生物信息学
作者
Wei Chen,Liang Gong,Zilong Guo,Wensheng Wang,Qian Zhang,Xianqing Liu,Sibin Yu,Lizhong Xiong,Jie Luo
出处
期刊:Molecular Plant
[Elsevier]
日期:2013-11-01
卷期号:6 (6): 1769-1780
被引量:1228
摘要
ABSTRACT
Liquid chromatography–mass spectrometry (LC–MS)-based metabolomics has been facilitated by the construction of MS2 spectral tag (MS2T) library from the total scan ESI MS/MS data, and the development of widely targeted metabolomics method using MS/MS data gathered from authentic standards. In this report, a novel strategy called stepwise multiple ion monitoring-enhanced product ions (stepwise MIM–EPI) was developed to construct the MS2T library, in which stepwise MIM was used as survey scans to trigger the acquisition of EPI. A total number of 698 (almost) non-redundant metabolites with MS2 spectra were obtained, of which 135 metabolites were identified/annotated. Integrating the data gathered from our MS2T library and other available multiple reaction monitoring (MRM) information, a widely targeted metabolomics method was developed to quantify 277 metabolites, including some phytohormones. Evaluation of the dehydration responses and natural variations of these metabolites in rice leaf not only suggested the coordinated regulation of abscisic acid (ABA) with metabolites such as serotonin derivative(s), polyamine conjugates under drought stress, but also revealed some C-glycosylated flavones as the potential markers for the discrimination of indica and japonica rice subspecies. The new MS2T library construction and widely targeted metabolomics strategy could be used as a tool for rice functional genomics. SUMMARY
A novel stepwise multiple ion monitoring–enhanced product ions (stepwise MIM–EPI) strategy was developed to construct the MS2T library. A widely targeted metabolomics method was adopted to quantify hundreds of metabolites simultaneously, and was applied to different biological processes.
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