三乙氧基硅烷
生物结合
化学
正硅酸乙酯
纳米颗粒
荧光
磁性纳米粒子
硅烷化
表面改性
荧光显微镜
纳米技术
组合化学
有机化学
材料科学
物理
物理化学
量子力学
作者
Jing Wu,Zhiqiang Ye,Guilan Wang,Jingli Yuan
出处
期刊:Talanta
[Elsevier]
日期:2007-07-31
卷期号:72 (5): 1693-1697
被引量:51
标识
DOI:10.1016/j.talanta.2007.03.018
摘要
Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties have been prepared by copolymerization of a conjugate of (3-aminopropyl)triethoxysilane bound to a fluorescent Eu3+ complex, 4,4′-bis(1″,1″,1″-trifluoro- 2″,4″-butanedion-4″-yl)chlorosulfo-o-terphenyl-Eu3+ (APS-BTBCT-Eu3+), free (3-aminopropyl)triethoxysilane (APS) and tetraethyl orthosilicate (TEOS) in the presence of poly(vinylpyrrolidone) (PVP) stabilized magnetic Fe3O4 nanoparticles (∼10 nm) with aqueous ammonia in ethanol. The nanoparticles were characterized by transmission electron microscopy (TEM), spectrofluorometry and vibrating sample magnetometry methods. The direct-introduced amino groups on the nanoparticle's surface by using free APS in nanoparticle preparation facilitated the surface modification and bioconjugation of the nanoparticles. The nanoparticle-labeled transferrin was prepared and used for staining the cultured Hela cells. A time-resolved fluorescence imaging technique that can fully eliminate the fast-decaying background noises was developed and used for the fluorescence imaging detection of the cells. A distinct image with the high ratio of signal to noise (S/N) was obtained.
科研通智能强力驱动
Strongly Powered by AbleSci AI