3D microscopy with phase-shifting digital holography

In this paper, we applied phase-shifting digital holography to microscopy. The digital holograms are recorded by a video camera from the superposition of a plane reference beam and the object beam passed through a microscopic objective. The distribution of complex amplitude of the object wave is derived by using the phase-shifting algorithm and then Fresnel transformed in a computer to reconstruct images at arbitrary planes. After relationships between object position and position of focused image and magnification are discussed, those are applied to determine the sizes and positions of small particles by image processing technique.