分生组织
异位表达
拟南芥
生物
拟南芥
细胞生物学
基因
植物
原基
突变体
生长素
开枪
遗传学
基因表达
作者
Xudong Sun,Zhenhua Feng,Lai-Sheng Meng,Jian Kang Zhu,Anja Geitmann
出处
期刊:Planta
[Springer Nature]
日期:2013-02-09
卷期号:237 (5): 1367-1378
被引量:29
标识
DOI:10.1007/s00425-013-1844-x
摘要
The ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (ASL/LBD) genes encode plant-specific nuclear proteins containing the conserved domain AS2/LOB. In this study, the function of a member of the Arabidopsis thaliana AS2/LOB gene family, ASL11/LBD15, was investigated. The results show that ASL11/LBD15 is expressed in the meristems of shoot apex, root apex, organ boundaries, and developing seeds. Overexpression of ASL11/LBD15 resulted in aberrant arrangements in the tunica cell layers of the shoot apical meristem (SAM). Two-week-old transgenic plants developed needle-like leaves in addition to regular leaves, while 6-week-old transformants displayed clustered cauline leaves suggesting altered SAM development. qRT-PCR analysis revealed that the WUSCHEL (WUS) transcript level was strongly up-regulated in plants overexpressing ASL11/LBD15 compared with the wild-type plants. Furthermore, inducible ASL11/LBD15 ectopic expression activated ectopic expression of WUS and affected the differentiation of leaf epidermal cells. Therefore, our results suggest that ASL11/LBD15 affects cellular differentiation in the SAM and regulates WUS expression.
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