腹膜
小干扰RNA
腹膜透析
肌成纤维细胞
腹膜腔
转化生长因子
纤维化
基因传递
体内
转染
药理学
癌症研究
医学
病理
化学
生物
内科学
解剖
基因
生物化学
生物技术
作者
Hiromichi Yoshizawa,Yoshiyuki Morishita,Megumi Watanabe,Kenichi Ishibashi,Shigeaki Muto,Eiji Kusano,Daisuke Nagata
出处
期刊:Gene Therapy
[Springer Nature]
日期:2015-01-08
卷期号:22 (4): 333-340
被引量:33
摘要
Gene therapies may be promising for the treatment of peritoneal fibrosis (PF) in subjects undergoing peritoneal dialysis (PD). However, a method of delivery of treatment genes to the peritoneum is lacking. We attempted to develop an in vivo small interfering RNA (siRNA) delivery system with liposome-based nanoparticles (NPs) to the peritoneum to inhibit PF. Transforming growth factor (TGF)-β1-siRNAs encapsulated in NPs (TGF-β1-siRNAs-NPs) dissolved in PD fluid were injected into the peritoneum of mice with PF three times a week for 2 weeks. TGF-β1-siRNAs-NPs knocked down TGF-β1 expression significantly in the peritoneum and inhibited peritoneal thickening with fibrous changes. TGF-β1-siRNAs-NPs also inhibited the increase of expression of α-smooth muscle actin-positive myofibroblasts. These results suggest that the TGF-β1-siRNA delivery system with NPs described here could be an effective therapeutic option for PF in subjects undergoing PD.
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