化学
催化作用
铑
酶
氧化还原
醇脱氢酶
电化学
脱氢酶
生物催化
酶催化
立体化学
药物化学
无机化学
有机化学
反应机理
物理化学
电极
作者
Maël Poizat,Isabel W. C. E. Arends,Frank Hollmann
出处
期刊:Journal of Molecular Catalysis B-enzymatic
[Elsevier]
日期:2010-05-01
卷期号:63 (3-4): 149-156
被引量:96
标识
DOI:10.1016/j.molcatb.2010.01.006
摘要
Pentamethylcyclopentadienyl rhodium bipyridine ([Cp*Rh(bpy)(H2O)]2+) is a versatile catalyst to promote biocatalytic redox reactions. However, its major drawback lies in the mutual inactivation of [Cp*Rh(bpy)(H2O)]2+ and the biocatalyst. This interaction was investigated using the alcohol dehydrogenase from Thermus sp. ATN1 (TADH) as model enzyme. TADH binds 4 equiv. of [Cp*Rh(bpy)(H2O)]2+ without detectable decrease in catalytic activity and stability. Higher molar ratios lead to time-, temperature-, and concentration-dependent inactivation of the enzyme suggesting [Cp*Rh(bpy)(H2O)]2+ to function as an ‘unfolding catalyst’. This detrimental activity can be circumvented using strongly coordinating buffers (e.g. (NH4)2SO4) while preserving its activity as NAD(P)H regeneration catalyst under electrochemical reaction conditions.
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