Study on Extracting TB from Liubao Tea with Cellulase Auxiliary Method

A new way using cellulase as auxiliary method to extract theabrownin (TB) from Guangxi Liubao Tea was proposed in this article. Single factor and orthogonal experiments were used to analysis the best extraction condition of TB from Tea. The effects of the enzyme dose, enzymatic hydrolysis time, enzymatic hydrolysis temperature and the PH of extraction were investigated by orthogonal test. Results showed that the order of influence factors was enzymatic hydrolysis time, the PH of extraction, enzyme dose and enzymatic hydrolysis temperature. The optimal extraction conditions were determined as follows: the dosage of cellulose 20 mg/g, the enzymatic hydrolysis time 40 min, the enzymatic hydrolysis temperature 30°C,the extraction PH=4. The highest extraction ration can attach 24.503%.