偶氮苯
磷酰胺
DNA
单体
化学
抄写(语言学)
部分
异构化
寡核苷酸
生物物理学
立体化学
生物化学
生物
聚合物
有机化学
语言学
哲学
催化作用
作者
Hiroyuki Asanuma,Xingguo Liang,H. Nishioka,Daijiro Matsunaga,Mingzhe Liu,Makoto Komiyama
出处
期刊:Nature Protocols
[Springer Nature]
日期:2007-01-01
卷期号:2 (1): 203-212
被引量:284
标识
DOI:10.1038/nprot.2006.465
摘要
A phosphoramidite monomer bearing an azobenzene is synthesized from D-threoninol. Using this monomer, azobenzene moieties can be introduced into oligodeoxyribonucleotide (DNA) at any position on a conventional DNA synthesizer. With this azobenzene-tethered DNA, formation and dissociation of a DNA duplex can be reversibly photo-regulated by cis-trans isomerization of the azobenzene. When the azobenzene takes a trans-form, a stable duplex is formed. After isomerization of the trans-azobenzene to its cis-form by UV-light irradiation (300 nm 400 nm). The introduction of azobenzenes into the T7 promoter at specific positions also efficiently and reversibly photo-regulates transcription by T7-RNA polymerase. The reversible regulation can be repeated many times without causing damage to the DNA or the azobenzene moiety. These procedures take approximately 10 d to complete.
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