Sequences 3’of Immunoglobulin Heavy Chain Genes Influence their Expression

分子生物学 生物 聚腺苷酸 转染 突变体 基因 抄写(语言学) 信使核糖核酸 外显子 基因表达 免疫球蛋白重链 核糖核酸 遗传学 语言学 哲学
作者
Polly D. Gregor,Barry J. Kobrin,Christine Milcarek,Sherie L. Morrison
出处
期刊:Immunological Reviews [Wiley]
卷期号:89 (1): 31-48 被引量:12
标识
DOI:10.1111/j.1600-065x.1986.tb01471.x
摘要

Immunological ReviewsVolume 89, Issue 1 p. 31-48 Sequences 3’of Immunoglobulin Heavy Chain Genes Influence their Expression Polly D. Gregor, Polly D. Gregor 1Department of Microbiology and the Cancer Center/Institute for Cancer Research, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, U.S.A.Search for more papers by this authorBarry J. Kobrin, Barry J. Kobrin 1Department of Microbiology and the Cancer Center/Institute for Cancer Research, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, U.S.A. Present Address: Department of Cell Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110, U.S.A.Search for more papers by this authorChristine Milcarek, Christine Milcarek 3 the Department of Microbiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, U.S.A.Search for more papers by this authorSherie L. Morrison, Sherie L. Morrison 1Department of Microbiology and the Cancer Center/Institute for Cancer Research, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, U.S.A.Search for more papers by this author Polly D. Gregor, Polly D. Gregor 1Department of Microbiology and the Cancer Center/Institute for Cancer Research, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, U.S.A.Search for more papers by this authorBarry J. Kobrin, Barry J. Kobrin 1Department of Microbiology and the Cancer Center/Institute for Cancer Research, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, U.S.A. Present Address: Department of Cell Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110, U.S.A.Search for more papers by this authorChristine Milcarek, Christine Milcarek 3 the Department of Microbiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, U.S.A.Search for more papers by this authorSherie L. Morrison, Sherie L. Morrison 1Department of Microbiology and the Cancer Center/Institute for Cancer Research, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, U.S.A.Search for more papers by this author First published: February 1986 https://doi.org/10.1111/j.1600-065X.1986.tb01471.xCitations: 7 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat REFERENCES Alt, F. W., Bothwell, A. L. W., Knapp, M., Siden, E., Mather, E., Koshland, M. & Baltimore, D. (1980) Synthesis of secreted and membrane-bound immunoglobulin Mu heavy chains is directed by mRNAs that differ at their 3′ ends. Cell 20, 293–301. 10.1016/0092-8674(80)90615-7 CASPubMedWeb of Science®Google Scholar Alt, F. W., Rosenberg, N., Enea, V., Siden, E. & Baltimore, D. (1982) Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines. Mol. Cell. Biol. 2, 386–400. 10.1128/MCB.2.4.386 CASPubMedWeb of Science®Google Scholar Alt, F. W., Rosenberg, N., Lewis, S., Thomas, E. & Baltimore, D. (1982) Organization and reorganization of immunoglobulin genes in A-MuLV-transformed cells: rearrangement of heavy but not light chain genes. Cell 27, 381–390. 10.1016/0092-8674(81)90421-9 Web of Science®Google Scholar Banerji, J., Olson, L. & Schaffner, W. (1983) A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. Cell 33, 729–740. 10.1016/0092-8674(83)90015-6 CASPubMedWeb of Science®Google Scholar Bentley, D. L., Farrell, P. J. & Rabbitts, T. H. (1982) Unrearranged immunoglobulin variable region genes have a functional promoter. Nucl. Acids Res. 10, 1841–1856. 10.1093/nar/10.6.1841 CASPubMedWeb of Science®Google Scholar Berk, A. J. & Sharp, P. A. (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of Si-nuclease digested hybrids. Cell 12, 721–732. 10.1016/0092-8674(77)90272-0 CASPubMedWeb of Science®Google Scholar Bogenhagen, D. F., Wormington, W. M. & Brown, D. D. (1982) Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state. Cell 28, 413–421. 10.1016/0092-8674(82)90359-2 CASPubMedWeb of Science®Google Scholar Brandt, C. R., Morrison, S. L., Birshtein, B. K. & Milcarek, C. (1984) Loss of a consensus splice signal in a mutant immunoglobulin gene eliminates the CH1 domain exon from the mRNA. Mol. Cell. Biol. 4 1270–1277. 10.1128/MCB.4.7.1270 CASPubMedWeb of Science®Google Scholar Breindl, M., Harbers, K. & Jaenisch, R. (1984) Retrovirus-induced lethal mutation in collagen I gene of mice is associated with an altered chromatin structure. Cell 38, 9–16. 10.1016/0092-8674(84)90521-X CASPubMedWeb of Science®Google Scholar Brown, D. D. (1984) The role of stable complexes that repress and activate eucaryotic genes. Cell 37, 359–365. 10.1016/0092-8674(84)90366-0 CASPubMedWeb of Science®Google Scholar Calame, K., Kim, S., Lalley, R, Hill, R., Davis, M. & Hood, L. (1982) Molecular cloning of translocations involving chromosome 15 and the immunoglobulin C gene from chromosome 12 in two murine plasmacytomas. Proc. Natl. Acad. Sci. U.S.A. 79, 6995–6998. 10.1073/pnas.79.22.6994 Web of Science®Google Scholar Church, G. M., Ephrussi, A., Gilbert, W. & Tonegawa, S. (1985) Cell-type-specific contacts to immunoglobulin enhancers in nuclei. Nature (London) 313, 798–800. 10.1038/313798a0 CASPubMedWeb of Science®Google Scholar Coffino, P. & Scharff, M. D. (1971) Rate of somatic mutation in immunoglobulin production by mouse myeloma cells. Proc. Natl. Acad. Sci. U.S.A. 68, 219–223. 10.1073/pnas.68.1.219 CASPubMedWeb of Science®Google Scholar Cole, M. D., Piccoli, S. P., Keath, E. J., Caimi, P & Kelekar, A. (1984) Activation of the myc oncogene by chromosomal translocation. In: Transfer and Expression of Eukaryotic Genes. H. S. Ginsberg & H. J. Vogel, eds. Academic Press, Inc., New York . Google Scholar Cooper, G. M. (1982) Cellular transforming genes. Science 217, 801–806. 10.1126/science.6285471 CASPubMedWeb of Science®Google Scholar Dackowski, W. & Morrison, S. L. (1981) Two a heavy chain disease proteins with different genomic deletions demonstrate that nonexpressed α heavy chain genes contain methylated bases. Proc. Natl. Acad. Sci. U.S.A. 78, 7091–7095. 10.1073/pnas.78.11.7091 CASPubMedWeb of Science®Google Scholar Dean, M., Kent, R. B. & Sonensheim, G. E. (1983) Transcriptional activation of immunoglobulin alpha heavy-chain genes by translocation of the c-myc oncogene. Nature (London) 305, 443–446. 10.1038/305443a0 CASPubMedWeb of Science®Google Scholar Early, D., Huang, H., Calame, K. & Hood, L. (1980) An immunoglobulin molecule heavy chain variable region is generated from three segments of DNA: VH, D and JH. Cell 19, 981–992. 10.1016/0092-8674(80)90089-6 CASPubMedWeb of Science®Google Scholar Eckhardt, L. A. & Birshtein, B. (1985) Independent immunoglobulin class-switch events occurring in a single myeloma cell line. Mol. Cell. Biol. 5, 856–868. 10.1128/MCB.5.4.856 CASPubMedWeb of Science®Google Scholar Ellison, J. & Hood, L. (1982) Linkage and sequence homology of two human immunoglobulin γ heavy chain constant region genes. Proc. Natl. Acad. Sci. U.S.A. 79, 1984–1988. 10.1073/pnas.79.6.1984 CASPubMedWeb of Science®Google Scholar Ephrussi, A., Church, G. M., Tonegawa, S. & Gilbert, W. (1985) Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult γ-globin gene: nature of the binding domain. Cell 41, 21–30. 10.1016/0092-8674(85)90057-1 PubMedWeb of Science®Google Scholar Falck-Pedersen, E., Logan, J., Shenk, T. & Darnell, J. E. Jr. (1985) Transcription termination within the El A gene of adenovirus induced by insertion of the mouse B-major globin terminator element. Cell 40, 897–905. 10.1016/0092-8674(85)90349-6 CASPubMedWeb of Science®Google Scholar Foster, J., Stafford, J. & Queen, C. (1985) An immunoglobulin promoter displays cell-type specificity independently of the enhancer. Nature (London) 315, 423–425. 10.1038/315423a0 CASPubMedWeb of Science®Google Scholar Gargiulo, G., Razvi, F. & Worcel, A. (1984) Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors. Cell 38, 511–521. 10.1016/0092-8674(84)90506-3 CASPubMedWeb of Science®Google Scholar Gillies, S. D, Morrison, S. L., Oi, V. T. & Tonegawa, S. (1983) A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene. Cell 33, 717–728. 10.1016/0092-8674(83)90014-4 CASPubMedWeb of Science®Google Scholar Grosschedl, R. & Baltimore, D. (1985) Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements. Cell 41, 885–897. 10.1016/S0092-8674(85)80069-6 CASPubMedWeb of Science®Google Scholar Hagenbuche, O., Wellauer, P. K., Cribbs, D. L. & Schibler, U. (1984) Termination of transcription in the mouse a-amylase gene Amy-2a occurs at multiple sites downstream of the polyadenylation site. Cell 38, 737–744. 10.1016/0092-8674(84)90269-1 PubMedWeb of Science®Google Scholar Harris, L. J., Lang, R. B. & Marcu, K. B. (1982) Non-immunoglobulin-associated DNA rearrangements in mouse plasmacytomas. Proc. Natl. Acad. Sci. U.S.A. 79, 4175–4179. 10.1073/pnas.79.13.4175 CASPubMedWeb of Science®Google Scholar Harvey, R. P, Robins, A. J. & Wells, J. R. E. (1982) Independently evolving chicken histone H2B genes: Identification of a ubiquitous H2B-specinc 5′ element. Nucl. Acid. Res. 10, 7851–7863. 10.1093/nar/10.23.7851 CASPubMedWeb of Science®Google Scholar Hawley, R. G., Shulman, M. J. & Hozumi, N. (1984) Transposition of two different intracisternal A particle elements into an immunoglobulin x-chain gene. Mol. Cell. Biol. 4, 2565–2572. 10.1128/MCB.4.12.2565 CASPubMedWeb of Science®Google Scholar Hayday, A. C, Gillies, S. D., Saito, H., Wood, C, Wiman, K., Hayward, W. S. & Tonegawa, S. (1984) Activation of a translocated human c-myc gene by an enhancer in the immunoglobulin heavy-chain locus. Nature (London) 307, 334–340. 10.1038/307334a0 CASPubMedWeb of Science®Google Scholar Kelly, K., Cochran, B. H., Stiles, C. D. & Leder, P. (1983) Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell 35, 603–610. 10.1016/0092-8674(83)90092-2 CASPubMedWeb of Science®Google Scholar Klein, S., Sablitzky, F. & Radbruch, A. (1984) Deletion of the IgH enhancer does not reduce immunoglobulin heavy chain production of a hybridoma IgD class switch variant. EMBO J. 3, 2473–2476. 10.1002/j.1460-2075.1984.tb02158.x CASPubMedWeb of Science®Google Scholar Laskov, R. & Scharff, M. D. (1970) Synthesis, assembly and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the MPC-11 tumor to culture cloning and characterization of gamma globulin subunits. J. Exp. Med. 131, 515–541. 10.1084/jem.131.3.515 CASPubMedGoogle Scholar Levitt, D & Cooper, M. D. (1980) Mouse pre-B cells synthesize and secrete α heavy chains but not light chains. Cell 19, 617–625. 10.1016/S0092-8674(80)80038-9 CASPubMedWeb of Science®Google Scholar Mason, J. O., Williams, G. T. & Neuberger, M. S. (1985) Transcription cell type specificity is conferred by an immunoglobulin VH gene promoter that includes a functional consensus sequence. Cell 41, 479–487. 10.1016/S0092-8674(85)80021-0 CASPubMedWeb of Science®Google Scholar Mather, E. L., Nelson, K. J., Haimovich, J. & Perry, R. P. (1984) Mode of regulation of immunoglobulin μ and δ-chain expression varies during B-lymphocyte maturation. Cell 36, 329–338. 10.1016/0092-8674(84)90226-5 CASPubMedWeb of Science®Google Scholar Mather, E. L. & Perry, R. P. (1981) Transcriptional regulation of immunoglobulin V genes. Nutl. Acids Res. 9, 6855–6867. 10.1093/nar/9.24.6855 CASPubMedWeb of Science®Google Scholar Max, E. E., Seidman, J. G. & Leder, P. (1979) Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene. Proc. Natl. Acad Sci. U.S.A. 76, 3450–3454. 10.1073/pnas.76.7.3450 CASPubMedWeb of Science®Google Scholar Melchers, F. & Andersson, J. (1984) B cell activation: three steps and their variations. Cell 37, 715–720. 10.1016/0092-8674(84)90407-0 CASWeb of Science®Google Scholar Melchers, F., Andersson, J., Lernhardt, W. & Schreier, M. H. (1980) Roles of surface-bound immunoglobulin molecules in regulating the replication and maturation to immunoglobulin secretion of B lymphocytes. Immunol. Rev. 52, 89–114. 10.1111/j.1600-065X.1980.tb00332.x CASPubMedWeb of Science®Google Scholar Mercola, M., Goverman, J., Mirell, C. & Calame, K. (1984) Immunoglobuiin heavy-chain enhancer requires one or more tissue-specific factors. Science 227, 266–270. 10.1126/science.3917575 PubMedWeb of Science®Google Scholar Milcarek, C. & Hall, B. (1985) Cell specific expression of secreted versus membrane forms of immunoglobulin gamma 2B mRNA involves selective use of alternate polyadenylation sites. Mol. Cell. Biol. 5, 2514–2520. 10.1128/MCB.5.10.2514 CASPubMedWeb of Science®Google Scholar Morrison, S. L. (1978) Murine heavy chain disease. Eur. J. Immunol. 8, 194–199. 10.1002/eji.1830080311 CASPubMedWeb of Science®Google Scholar Nelson, K. J., Haimovich, J. & Perry, R. P. (1983) Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: processing of μm and μs mRNA. Molec. Cell. Biol. 3, 1317–1332. 10.1128/MCB.3.7.1317 CASPubMedWeb of Science®Google Scholar Nossal, G. J. V. & Pike, B. L. (1972) Differentiation of B lymphocytes from stem cell precursors. Adv. Exp. Med. Biol. 29, 11–20. 10.1007/978-1-4615-9017-0_3 Google Scholar Okamura, K., Julius, M. H., Herzenberg, L. & Herzenberg, L. A. (1976) Demonstration that IgG memory is carried by IgG-bearing cells. J. Immunol. 6, 467–472. Google Scholar Paige, C. J., Kincade, P. W. & Ralph, P. (1981) Independent control of immunoglobulin heavy and light chain expression in a murine pre-B-cell line. Nature (London) 292, 631–633. 10.1038/292631a0 CASPubMedWeb of Science®Google Scholar Parslow, T. G., Blair, D. L., Murphy, W. J. & Granner, D. K. (1984) Structure of the 5′ ends of immunoglobulin genes: A novel conserved sequence. Proc. Natl. Acad. Sci. U.S.A. 81, 2650–2654. 10.1073/pnas.81.9.2650 CASPubMedWeb of Science®Google Scholar Parslow, T. G. & Granner, D. K. (1983) Structure of a nuclease-sensitive region inside the immunoglobulin x gene: evidence for a role in gene regulation. Nucl. Acid. Res. 11, 4775–4792. 10.1093/nar/11.14.4775 CASPubMedWeb of Science®Google Scholar Perry, R. P. & Kelley, D. E. (1979) Immunoglobulin messenger RNAs in murine cell lines that have characteristics of immature B lymphocytes. Cell 18, 1333–1339. 10.1016/0092-8674(79)90243-5 CASPubMedWeb of Science®Google Scholar Perry, R. P, Kelley, D. E., Coleclough, C. & Kearney, J. F. (1981) Organization and expression of immunoglobulin genes in fetal liver hybridomas. Proc. Natl. Acad. Sci. U.S.A. 78, 247–251. 10.1073/pnas.78.1.247 CASPubMedWeb of Science®Google Scholar Perry, R. P, Kelley, D. E., Coleclough, C, Seidman, J. G., Leder, P, Tonegawa, S., Matthyssens, G. & Weigert, M. (1980) Transcription of mouse kappa chain genes: implications for allelic exclusion. Proc. Natl. Acad. Sci. U.S.A. 11, 1937–1941. 10.1073/pnas.77.4.1937 Web of Science®Google Scholar Picard, D. & Schaffner, W. (1984) A lymphocyte-specific enhancer in the mouse immunoglobulin kappa gene. Nature (London) 307, 80–82. 10.1038/307080a0 CASPubMedWeb of Science®Google Scholar Piccoli, S. P., Caimi, P. G. & Cole, M. D. (1984) A conserved sequence at c-myc oncogene chromosomal translocation breakpoints in plasmacytomas. Nature (London) 31, 327–330. 10.1038/310327a0 Web of Science®Google Scholar Raff, M. C, Megson, M., Owen, J. J. T. & Cooper, M. D. (1976) Early production of intracellular IgM by B-lymphocyte precursors in mouse. Nature (London) 259, 224–226. 10.1038/259224a0 CASPubMedGoogle Scholar Richards, J. E., Gilliam, A. C, Shen, A., Tucker, P. W. & Blattner, F. R. (1983) Unusual sequences in the murine immunoglobulin μ-δ heavy-chain region. Nature (London) 306, 483–487. 10.1038/306483a0 CASPubMedWeb of Science®Google Scholar Rogers, J., Choi, E., Souza, S., Carter, C, Word, C, Kuehl, M., Eisenberg, D. & Wall, R. (1981) Gene segments encoding transmembrane carboxyl termini of immunoglobulin gamma chains. Cell 26, 19–27. 10.1016/0092-8674(81)90029-5 CASPubMedWeb of Science®Google Scholar Sakano, H., Rogers, J. H., Huppi, K., Brack, C, Traunecker, A., Maki, R., Wall, R. & Tonegawa, S. (1979) Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments. Nature (London) 277, 627–633. 10.1038/277627a0 CASPubMedWeb of Science®Google Scholar Schibler, U., Marcu, K. B. & Perry, R. P (1978). The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells. Cell. 15, 1495–1509. 10.1016/0092-8674(78)90072-7 CASPubMedWeb of Science®Google Scholar Schlissel, M. S. & Brown, D. D. (1984) The transcriptional regulation of Xenopus 5S RNA genes in chromatin: the roles of active stable transcription complexes and histone HI. Cell 37, 903–913. 10.1016/0092-8674(84)90425-2 CASPubMedWeb of Science®Google Scholar Shen-Ong, G. L. C, Keath, E. J., Piccoli, S. P & Cole, M. D. (1982) Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas. Cell 31, 443–452. 10.1016/0092-8674(82)90137-4 PubMedWeb of Science®Google Scholar Siden, E., Alt, F. W., Shinefeld, L., Sato, V. & Baltimore, D. (1981) Synthesis of immunoglobulin μ chain gene products precedes synthesis of light chains during B-lymphocyte development. Proc. Natl. Acad. Sci. U.S.A. 78, 1823–1827. 10.1073/pnas.78.3.1823 CASPubMedWeb of Science®Google Scholar Siebenlist, U., Ravetch, J. V., Korsmeyer, S., Waldmann, T. & Leder, P (1981) Human immunoglobulin D segments encoded in tandem multigenic families. Nature (London) 294, 631–635. 10.1038/294631a0 CASPubMedWeb of Science®Google Scholar Stanton, L. W., Yang, J., Eckhardt, L. A., Harris, L. J., Birshtein, B. K. & Marcu, K. B. (1984) Products of a reciprocal chromosome translocation involving the c-myc gene in a murine plasmacytoma. Proc. Natl. Acad. Sci. U.S.A. 81, 829–833. 10.1073/pnas.81.3.829 CASPubMedWeb of Science®Google Scholar Taub, R., Kirsch, I., Morton, C, Lenoir, G., Swan, D., Tronick, S., Aaronson, S. & Leder, P. (1982) Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. U.S.A. 19, 7837–7841. 10.1073/pnas.79.24.7837 CASWeb of Science®Google Scholar Tyler, B. M., Cowman, A. F., Gerondakis, S. D., Adams, J. M. & Bernard, O. (1982) mRNA for surface immunoglobulin γ chains encodes a highly conserved transmembrane sequence and a 28-residue intracellular domain. Proc. Natl. Acad. Sci. U.S.A. 79, 2008–2012. 10.1073/pnas.79.6.2008 CASPubMedWeb of Science®Google Scholar Van Ness, B. G., Weigert, M., Coleclough, C, Mather, E. L., Kelley, D. E. & Perry, R. P. (1981) Transcription of the unrearranged mouse Ck locus: sequence of the initiation region and comparison of activity with a rearranged Vk-Ckgene. Cell 27, 593–601. 10.1016/0092-8674(81)90401-3 PubMedWeb of Science®Google Scholar Villerreal, L. P & White, R. T. (1983) A splice junction deletion deficient in the transport of RNA does not polyadenylate nuclear RNA. Molec. Cell. Biol. 3, 1381–1388. 10.1128/MCB.3.8.1381 Google Scholar Weis, J. A., Word, C. J., Rimm, D., Der-Balan, G. P, Martinez, H. M., Tucker, P. W. & Blattner, F. R. (1984) Structural analysis of the murine IgG3 constant region gene. EMBO J. 3, 2041–2046. PubMedWeb of Science®Google Scholar Word, C. J. & Kuehl, W. M. (1981) Expression of surface and secreted IgG2a by a murine B-lymphoma before and after hybridization to myeloma cells. Mol. Immunol. 18, 311–322. 10.1016/0161-5890(81)90055-9 CASPubMedWeb of Science®Google Scholar Yancopoulos, G. D. & Alt, F. W. (1985) Developmentally controlled and tissue-specific expression of unrearranged Vngene segments. Cell 40, 271–281. 10.1016/0092-8674(85)90141-2 CASPubMedWeb of Science®Google Scholar Zaller, D. M. & Eckhardt, L. A. (1985) Deletion of a B-cell-specific enhancer affects transfected, but not endogenous, immunoglobulin heavy-chain gene expression. Proc. Natl. Acad. Sci. U.S.A. 82, 5088–5092. 10.1073/pnas.82.15.5088 CASPubMedWeb of Science®Google Scholar Citing Literature Volume89, Issue1February 1986Pages 31-48 ReferencesRelatedInformation

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