Gene therapy using non-viral peptide vector in a canine systemic lupus erythematosus model

免疫系统 生物 遗传增强 病毒载体 免疫学 抗体 病毒学 载体(分子生物学) 基因 重组DNA 生物化学
作者
Eun Wha Choi,Ilhoon Shin,Hwa‐Young Youn,Dae-Yong Kim,Hang Lee,Young-Jin Chae,Chang-Woo Lee
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier BV]
卷期号:103 (3-4): 223-233 被引量:13
标识
DOI:10.1016/j.vetimm.2004.09.027
摘要

Although viral vectors are commonly used for therapeutic gene delivery, their applications are limited due to their specific cell membrane receptor-mediated infection and host immune response. In the present study, we constructed a non-viral peptide vector and applied it in the treatment of experimentally induced systemic lupus erythematosus-like disease in dogs. For therapeutic gene construction, the extracellular domain of canine CTLA-4, and the CH2–CH3 domains of canine immunoglobulin alpha constant region were inserted between the cytomegalovirus promoter and poly-adenylation sequence of bovine growth hormone. The constructed therapeutic gene was ligated to the non-viral synthetic peptide vector and was applied to systemic lupus erythematosus-like disease induced dogs. After gene therapy, clinical signs of systemic lupus erythematosus were reduced dramatically: the anti-nuclear antibody titers and urine protein/creatinine ratios were recovered to normal values, and the skin regained its normal histological features. The peptide vector did not show either tissue specific tropism or host induced immune response.
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