Bacteria clustering by polymers induces the expression of quorum-sensing-controlled phenotypes

Bacteria deploy a range of chemistries to regulate their behaviour and respond to their environment. Quorum sensing is one method by which bacteria use chemical reactions to modulate pre-infection behaviour such as surface attachment. Polymers that can interfere with bacterial adhesion or the chemical reactions used for quorum sensing are therefore a potential means to control bacterial population responses. Here, we report how polymeric 'bacteria sequestrants', designed to bind to bacteria through electrostatic interactions and therefore inhibit bacterial adhesion to surfaces, induce the expression of quorum-sensing-controlled phenotypes as a consequence of cell clustering. A combination of polymer and analytical chemistry, biological assays and computational modelling has been used to characterize the feedback between bacteria clustering and quorum sensing signalling. We have also derived design principles and chemical strategies for controlling bacterial behaviour at the population level. Bacteria use signalling networks to control population behaviour. Here it is shown that polymers that promote clustering of bacteria can influence bacterial signalling and induce unexpected responses in quorum-sensing-controlled phenotypes. These responses can be better modulated by controlling the affinity of the polymer to both bacteria and signalling molecules.