胶体金
纳米颗粒
聚电解质
阳离子聚合
亚乙基亚胺
动态光散射
材料科学
表面改性
表面等离子共振
纳米技术
基因传递
电泳
聚合物
药物输送
组合化学
化学工程
化学
高分子化学
转染
色谱法
生物化学
工程类
基因
复合材料
作者
Geraldine Genevive Lazarus,Neerish Revaprasadu,J. López-Viota,Moganavelli Singh
标识
DOI:10.1016/j.colsurfb.2014.06.032
摘要
Gold nanoparticles have attracted strong biomedical interest for drug delivery due to their low toxic nature, surface plasmon resonance and capability of increasing the stability of the payload. However, gene transfection represents another important biological application. Considering that cellular barriers keep enclosed their secret to deliver genes using nanoparticles, an important step can be achieved by studying the functionalization of nanoparticles with DNA. In the present contribution the synthesis of nanoparticles consisting of a gold core coated with one or more layers of amino acid (l-lysine), and cationic polyelectrolytes (poly-ethyleneimine and poly-l-lysine) is reported. All nanoparticles were subjected to dynamic light scattering, electrophoretic mobility measurements, UV–vis optical spectrophotometry analysis and transmission electron microscopy imaging. In addition, the adsorption of DNA plasmid (pSGS) with linear and supercoiled configurations was studied for those gold nanoparticles under the most suitable surface modifications. Preliminary results showed that the gold nanoparticles functionalized with poly-ethyleneimine and poly-l-lysine, respectively, and bound to linear DNA configurations, present in absolute value a higher electrophoretic mobility irrespective of the pH of the media, compared to the supercoiled and nicked configuration. The findings from this study suggest that poly-ethyleneimine and poly-l-lysine functionalized gold nanoparticles are biocompatible and may be promising in the chemical design and future optimization of nanostructures for biomedical applications such as gene and drug delivery.
科研通智能强力驱动
Strongly Powered by AbleSci AI