DNA methylation pattern of apoptosis‐related genes in ameloblastoma

成釉细胞瘤 甲基化 DNA甲基化 基因 生物 分子生物学 基因表达 实时聚合酶链反应 发起人 抄写(语言学) 聚合酶链反应 细胞凋亡 遗传学 解剖 上颌骨 语言学 哲学
作者
S.R.C.F. Costa,Pereira Nb,Karuza Maria Alves Pereira,KF Campos,WH Castro,MG Diniz,Carolina Cavaliéri Gomes,Ricardo Santiago Gomez
出处
期刊:Oral Diseases [Wiley]
卷期号:23 (6): 779-783 被引量:16
标识
DOI:10.1111/odi.12661
摘要

Objectives DNA methylation is an important mechanism of gene control expression, and it has been poorly addressed in odontogenic tumours. On this basis, we aimed to assess the methylation pattern of 22 apoptosis‐related genes in solid ameloblastomas. Materials and Methods Ameloblastoma fresh samples ( n = 10) and dental follicles ( n = 8) were included in the study. The percentage fraction of methylated and unmethylated DNA promoter of 22 apoptosis‐related genes was determined using enzymatic restriction digestion and quantitative real‐time PCR ( qPCR ) array. The relative expressions of the genes that showed the most discrepant methylation profile between tumours and controls were analysed by reverse‐transcription quantitative PCR ( RT ‐ qPCR ). Results Lower methylation percentages of TNFRSF 25 (47.2%) and BCL 2L11 (33.2%) were observed in ameloblastomas compared with dental follicles (79.3% and 59.5%, respectively). The RT ‐ qPCR analysis showed increased expression of BCL 2L11 in ameloblastomas compared with dental follicles, in agreement with the methylation analysis results, while there was no difference between the expression levels of TNFRSF 25 between both groups. Conclusions On the basis of our results, the transcription of the apoptosis‐related gene BCL 2L11 is possibly regulated by promoter DNA methylation in ameloblastoma. The biological significance of this finding in ameloblastoma pathobiology remains to be clarified.
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