生物发光
荧光素
荧光素酶
生物发光成像
萤火虫协议
光蛋白
生物物理学
萤光素酶类
体内
化学
生物化学
生物
转染
动物
基因
生物技术
作者
Takahiro Kuchimaru,Satoshi Iwano,Masahiro Kiyama,Shun Mitsumata,Tetsuya Kadonosono,Haruki Niwa,Shôjirô Maki,Shinae Kizaka‐Kondoh
摘要
In preclinical cancer research, bioluminescence imaging with firefly luciferase and D-luciferin has become a standard to monitor biological processes both in vitro and in vivo. However, the emission maximum (λmax) of bioluminescence produced by D-luciferin is 562 nm where light is not highly penetrable in biological tissues. This emphasizes a need for developing a red-shifted bioluminescence imaging system to improve detection sensitivity of targets in deep tissue. Here we characterize the bioluminescent properties of the newly synthesized luciferin analogue, AkaLumine-HCl. The bioluminescence produced by AkaLumine-HCl in reactions with native firefly luciferase is in the near-infrared wavelength ranges (λmax=677 nm), and yields significantly increased target-detection sensitivity from deep tissues with maximal signals attained at very low concentrations, as compared with D-luciferin and emerging synthetic luciferin CycLuc1. These characteristics offer a more sensitive and accurate method for non-invasive bioluminescence imaging with native firefly luciferase in various animal models.
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