Determination of loureirin A and loureirin B in Dracaena cochinchinensis by substitute reference substance

Objective: To establish an HPLC method for determination of loureirin A and loureirin B in Dracaena cochinchinensis by substitute reference substance.Method: To give the definition and physical interpretation of relative slope(RS),explain the relationship between RS and relative correction factor.The determination of loureirin A and loureirin B was established and validated.Results: The quantitative analysis was demonstrated with good linear relationship and recoveries.The RS and relative retention time of loureirin B detected by five different HPLC systems showed good repeatabilities.And mean values were 1.248 and 1.06.The values of RS detected by HPLC and ultraviolet spectrophotometry were equal.Conclusion: The method is simple and accurate,can be used for determination of loureirin A and loureirin B in D.cochinchinensis.