葡萄糖氧化酶
超顺磁性
材料科学
热稳定性
纳米复合材料
生物传感器
化学工程
固定化酶
还原胺化
纳米技术
透射电子显微镜
纳米颗粒
粒径
纳米-
磁性纳米粒子
化学
磁化
复合材料
有机化学
磁场
催化作用
酶
工程类
物理
量子力学
作者
Xianxiang Wang,Shuo Huang,Zhi Shan,Wanjun Yang
标识
DOI:10.1007/s11434-009-0113-7
摘要
Superparamagnetism amination nanocrystals Fe3O4 with 3-aminopropyltriethyloxy silane (APTES) were prepared by modified co-precipitation method. Next, 4-5 nm gold nanoparticles, prepared by classical Frens procedure, were coated on the surface of the amination Fe3O4 by self-assembly technology. The prepared Fe3O4@Au nanocomposite particles were investigated by transmission electron microscopy (TEM), UV-vis, infrared spectrum (FT-IR), and vibrating sample magnetometer (VSM) in order to elucidate the morphology, optics and magnetic properties of the nanocomposites. Their uniform distribution of particle size, which is about 15 nm, and good magnetic responsiveness were observed. In view of the fact that Fe3O4 owns superparamagnetism and that nano-gold can readily combine with biological molecules, glucose oxidase (GO x ) was chosen as a model to penetrate the condition of immobilizing enzyme, and enzymatic properties of resultant immobilized enzyme were studied as well. By systematic optimization, we established that at 28°C, and pH (5.5) and when mole ratio of Fe3O4:HAuCl4 was 0.5:1, the immobilization provided the best results. Finally, we are glad to find that the immobilized enzyme exhibits excellent thermostability in addition to its better stability than free enzyme. Thus, herein described immobilized enzyme could be used repeatedly with the assistance of an external magnetic field.
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