Purification of an acidic polysaccharide from Sarcandra glabra and its inhibitory effect on α-glycosidase activity

Through the preparation of the crude polysaccharide( SGP-C) from Sarcandra glabra( Thunb)Nakai,the polysaccharide of SGP-O was obtained with optimal purification conditions( pH 9,5% H2O2,50 °C,decolored for 3. 5 h). Then, high-quality polysaccharide( SGP-2) was purified by Sephacryl S-400 molecular sieve with the yield of 0. 35%. In vitro α-glucosidase inhibition assay indicated that SGP-2 had an IC50of( 87. 06 ±11. 76) μg /mL,only a quarter of that of acarbose( 338. 9 ± 46. 86 μg /mL),which indicated that SGP-2 had strong α-glucosidase enzyme inhibitory activity. Preliminary dynamic studies showed that SGP-2 inhibited α-glucosidase in a reversible and mixed-type mode.