透明质酸
化学
体内
色谱法
透皮
间质液
药理学
病理
医学
遗传学
生物技术
生物
作者
Dan Zhu,Le Wen Zheng,Phan Khanh Duong,Reanne Hui Cheah,Xin Yi Liu,Jun Ren Wong,Wenjun Wang,Steven Tien Guan Thng,Xin Ting Zheng,Peng Chen
标识
DOI:10.1016/j.bios.2022.114412
摘要
Skin Interstitial Fluid (ISF) is an alternative source for biomarkers. Herein, a highly swellable microneedle patch (MNP) to rapidly extract ISF painlessly and bloodlessly is presented. The MNP is made of crosslinked methacrylated hyaluronic acid (MeHA) and dissolvable hyaluronic acid (HA) with the optimal balance of mechanical strength (0.6 N/MN) and absorption capability (16.22 μL in 20 min). Incorporated with wax-patterned and sensing-reagent-decorated test paper (TP) for multiplexed colorimetric detection of metabolites (glucose, lactate, cholesterol, and pH), this TP-MNP biosensor gives rapid color change in biomarker concentration-dependent manner based on specific enzymatic reactions, whereby allowing diagnosis by the naked eye or quantitative RGB analysis. Both the in vitro and in vivo experiments demonstrate the feasibility of TP-MNPs to detect multiple biomarkers in skin interstitial fluid within minutes. Such convenient and self-administrable profiling of metabolites shall be instrumental for home-based long-term monitoring and management of metabolic diseases.
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