Expression of cartilage oligomeric matrix protein (COMP) associates with capsular invasion in salivary gland pleomorphic adenoma

小桶 下调和上调 唾液腺 细胞外基质 癌变 生物 软骨寡聚基质蛋白 多形性腺瘤 基因 免疫组织化学 基因表达 癌症研究 病理 分子生物学 细胞生物学 医学 遗传学 免疫学 基因本体论 骨关节炎 替代医学
作者
Xiu Yao,Hua Guan,Yanjun Lin,Yan Li,Yanjing Ou,Mingdong Yan,Lisong Lin,Xiaofeng Zhu,Bin Shi,Jiang Chen
出处
期刊:Journal of Oral Pathology & Medicine [Wiley]
卷期号:51 (3): 290-300
标识
DOI:10.1111/jop.13277
摘要

Accumulating evidence shows that pleomorphic adenoma (PA) exhibits a unique capsular invasion and with a crucial role in recurrence. This study was designed to explore RNA expression profiles in salivary gland PA in an attempt to further analyse genes associate with capsule invasion.We evaluated the expression profiles of 4 salivary gland PA patients by RNA-sequencing. The principal functions of the differentially expressed mRNAs (DEGs) were explored using GO and KEGG analysis. Then, RT-qPCR and correlation analyses were performed to verify the candidate DEGs in 59 PA patients, and immunohistochemical examinations were conducted to validate candidate DEGs. Finally, the COMP-related genes were screened using correlation and biological pathway enrichment analysis, and further validated by RT-qPCR.A total of 974 DEGs were significantly upregulated, and 1464 were downregulated (fold change ≥2.0; p < 0.05). Based on GO and KEGG analyses, extracellular matrix organization and the PI3K-Akt signalling pathway might play pivotal roles in the tumorigenesis of PA. 40 DEGs were screened and validated by RT-qPCR, 11 upregulated and 5 downregulated DEGs were consistent with the sequencing results. Cartilage oligomeric matrix protein (COMP) was identified to have a significant correlation with the capsular invasion of PA and expression of COMP in patients with invasive capsular PA was significantly stronger than PA. Finally, further results could reveal that 5 highest scoring genes were screened as hub genes for COMP.These findings suggested that COMP may be a prognostic target for PA and might contribute to its capsular invasion.
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