HPLC-MS/MS测定大鼠血浆及组织中盐酸戊乙奎醚浓度

色谱法 化学 高效液相色谱法 醋酸铵 甲酸 石油醚 萃取(化学) 三乙胺 乙醚 有机化学
作者
余勤,梁茂植,南峰,向瑾,秦永平
摘要

Objective To develop a method for detecting penehyclidine hydrochloride (PH) in mouse plasma and tissues using HPLC-MS/MS. Methods The plasma and tissue samples were extracted with petroleum ether: ethyl ether (70: 30). The isolation of PH was achieved on an Allure C18 (50×2.1 mm, 5μm) column, with methanol/5 mmol/L ammonium acetate/triethylamine=90/10/0.05 (adjusted pH5.8 by formic acid as mobile phase. Benzhydramine was used as the internal standard. Results The calibration curves were linear over the range of 0.39-200 ng/mL for plasma and 0.391-100 ng/mL for tissues. The extraction recovered 67.32%-70.80% of PH in plasma, 84.99%-89.27% of PH in lung tissues, and 76.86%-81.98% of PH in brain tissues. The HPLC detected 97.66%-99.97% of PH in extracted samples of plasma, 96.55%-101.65% extracted samples of lung and 95.18%-100.21% of extracted samples of brain. The within-day RSD were 1.94%-2.93%, 1.73%-3.70% and 2.35%-2.79% for plasma, lung, and brain, respectively. The between-day RSD were 3.00%-3.85% 2.86%-3.94% and 2.77%-5.00% for plasma, lung, and brain, respectively. Both the long-term and freeze-thaw stabilities were acceptable. The long-term RSD were 1.52%-5.26%, 1.88%-2.93%, and 2.22%-3.76% for plasma, lung, and brain, respectively. The freeze-thaw RSD were 0.38%-3.55%, 2.79%-9.60%, and 1.35%-2.29% for plasma, lung, and brain, respectively. Conclusion The assay is simple and accurate, with good reproducibility, and can satisfy to the needs of pharmacokinetics and relative bioavailability studies on penehyclidine hydrochloride.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
小二郎应助孜然炸鸡排采纳,获得30
1秒前
隐形曼青应助TJJJJJ采纳,获得10
1秒前
Ava应助明亮小甜瓜采纳,获得10
1秒前
西西发布了新的文献求助10
1秒前
完美世界应助mariawang采纳,获得10
2秒前
2秒前
遐蝶发布了新的文献求助10
2秒前
wangjing11完成签到,获得积分10
3秒前
轻松小张发布了新的文献求助900
3秒前
机智采枫完成签到 ,获得积分10
3秒前
脑洞疼应助云栖采纳,获得10
3秒前
峥2发布了新的文献求助10
4秒前
4秒前
5秒前
5秒前
6秒前
6秒前
7秒前
7秒前
lm发布了新的文献求助10
8秒前
8秒前
8秒前
zhao完成签到,获得积分10
8秒前
weiwei完成签到,获得积分10
8秒前
科研通AI2S应助姜凯采纳,获得10
9秒前
孙福禄应助西西采纳,获得10
10秒前
孙福禄应助西西采纳,获得10
10秒前
10秒前
单纯青槐发布了新的文献求助10
10秒前
10秒前
10秒前
luckbaby完成签到,获得积分10
11秒前
方赫然发布了新的文献求助10
11秒前
JerryZ发布了新的文献求助10
11秒前
今后应助高贵绿真采纳,获得10
11秒前
Ava应助mariawang采纳,获得10
11秒前
11秒前
菜不透发布了新的文献求助10
12秒前
12秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988786
求助须知:如何正确求助?哪些是违规求助? 3531116
关于积分的说明 11252493
捐赠科研通 3269766
什么是DOI,文献DOI怎么找? 1804771
邀请新用户注册赠送积分活动 881870
科研通“疑难数据库(出版商)”最低求助积分说明 809021