Hindsight regulates photoreceptor axon targeting through transcriptional control of jitterbug/Filamin and multiple genes involved in axon guidance in Drosophila

菲拉明 生物 轴突引导 轴突 转录因子 细胞生物学 遗传学 FLNA公司 转录调控 基因 神经科学 细胞骨架 细胞
作者
Carlos Oliva,Claudia Molina-Fernández,Miguel Maureira,Noemí Candia,Estefanía López,Bassem A. Hassan,Stein Aerts,José Cánovas,Patricio Olguı́n,Jimena Sierralta
出处
期刊:Developmental Neurobiology [Wiley]
卷期号:75 (9): 1018-1032 被引量:18
标识
DOI:10.1002/dneu.22271
摘要

During axon targeting, a stereotyped pattern of connectivity is achieved by the integration of intrinsic genetic programs and the response to extrinsic long and short-range directional cues. How this coordination occurs is the subject of intense study. Transcription factors play a central role due to their ability to regulate the expression of multiple genes required to sense and respond to these cues during development. Here we show that the transcription factor HNT regulates layer-specific photoreceptor axon targeting in Drosophila through transcriptional control of jbug/Filamin and multiple genes involved in axon guidance and cytoskeleton organization.Using a microarray analysis we identified 235 genes whose expression levels were changed by HNT overexpression in the eye primordia. We analyzed nine candidate genes involved in cytoskeleton regulation and axon guidance, six of which displayed significantly altered gene expression levels in hnt mutant retinas. Functional analysis confirmed the role of OTK/PTK7 in photoreceptor axon targeting and uncovered Tiggrin, an integrin ligand, and Jbug/Filamin, a conserved actin- binding protein, as new factors that participate of photoreceptor axon targeting. Moreover, we provided in silico and molecular evidence that supports jbug/Filamin as a direct transcriptional target of HNT and that HNT acts partially through Jbug/Filamin in vivo to regulate axon guidance. Our work broadens the understanding of how HNT regulates the coordinated expression of a group of genes to achieve the correct connectivity pattern in the Drosophila visual system. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 75: 1018-1032, 2015.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
舒适亦凝发布了新的文献求助10
刚刚
啰友痕武次子完成签到,获得积分10
1秒前
1秒前
大个应助djx123采纳,获得10
1秒前
slowstar完成签到,获得积分10
2秒前
linkr5发布了新的文献求助10
2秒前
3秒前
3秒前
Steve发布了新的文献求助10
4秒前
丘比特应助栀盎采纳,获得30
4秒前
笑点低白秋完成签到,获得积分10
5秒前
7秒前
7秒前
7秒前
852应助Li采纳,获得10
8秒前
南天一柱完成签到,获得积分10
8秒前
9秒前
9秒前
一只奶茶派完成签到 ,获得积分10
9秒前
whf发布了新的文献求助10
10秒前
上官若男应助闫奥采纳,获得10
11秒前
芯止谭轩发布了新的文献求助20
11秒前
可靠秋柳完成签到,获得积分10
12秒前
汉堡包应助唱跳双c采纳,获得10
12秒前
xibaluma发布了新的文献求助10
12秒前
Steve完成签到,获得积分10
12秒前
李新祎发布了新的文献求助10
13秒前
djx123发布了新的文献求助10
13秒前
Hello应助呵浅陌采纳,获得10
13秒前
野花做了玫瑰花的梦完成签到,获得积分10
13秒前
tangli完成签到 ,获得积分10
14秒前
李爱国应助fanicky采纳,获得10
14秒前
吟游诗人发布了新的文献求助10
14秒前
路人完成签到,获得积分10
14秒前
Lucas应助BPX采纳,获得30
15秒前
CipherSage应助BPX采纳,获得10
15秒前
17秒前
17秒前
科研通AI2S应助1234采纳,获得10
17秒前
17秒前
高分求助中
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
The Heath Anthology of American Literature: Early Nineteenth Century 1800 - 1865 Vol. B 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
Machine Learning for Polymer Informatics 500
《关于整治突出dupin问题的实施意见》(厅字〔2019〕52号) 500
2024 Medicinal Chemistry Reviews 480
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3221384
求助须知:如何正确求助?哪些是违规求助? 2870168
关于积分的说明 8169192
捐赠科研通 2536983
什么是DOI,文献DOI怎么找? 1369208
科研通“疑难数据库(出版商)”最低求助积分说明 645386
邀请新用户注册赠送积分活动 619051