Novel Insights Into Appropriate Encapsulation Methods for Bioactive Compounds Into Polymers: A Study With Peptides and HDAC Inhibitors

Macromolecular BioscienceVolume 14, Issue 1 p. 69-80 Full Paper Novel Insights Into Appropriate Encapsulation Methods for Bioactive Compounds Into Polymers: A Study With Peptides and HDAC Inhibitors† Dorle Hennig, Dorle Hennig Center for Molecular Biomedicine, Institute of Biochemistry and Biophysics, Department of Biochemistry, Friedrich Schiller University Jena, Hans-Knöll-Str. 2, D-07745 Jena, GermanySearch for more papers by this authorStephanie Schubert, Stephanie Schubert Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany Department of Pharmaceutical Technology, Friedrich Schiller University, JenaLessingstr. 8, D-07743 Jena, GermanySearch for more papers by this authorHarald Dargatz, Harald Dargatz Institute of Pharmaceutical Biology, Rheinische Friedrich Wilhelms University, BonnNussallee 6, D-53115 Bonn, GermanySearch for more papers by this authorEvi Kostenis, Evi Kostenis Institute of Pharmaceutical Biology, Rheinische Friedrich Wilhelms University, BonnNussallee 6, D-53115 Bonn, GermanySearch for more papers by this authorAlfred Fahr, Alfred Fahr Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany Department of Pharmaceutical Technology, Friedrich Schiller University, JenaLessingstr. 8, D-07743 Jena, GermanySearch for more papers by this authorUlrich S. Schubert, Ulrich S. Schubert Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University, JenaHumboldtstr. 10, D-07743 Jena, GermanySearch for more papers by this authorThorsten Heinzel, Thorsten Heinzel Center for Molecular Biomedicine, Institute of Biochemistry and Biophysics, Department of Biochemistry, Friedrich Schiller University Jena, Hans-Knöll-Str. 2, D-07745 Jena, Germany Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, GermanySearch for more papers by this authorDiana Imhof, Corresponding Author Diana Imhof Pharmaceutical Chemistry I, Institute of Pharmacy, Rheinische Friedrich Wilhelms University, BonnBrühler Str. 7, D-53119 Bonn, Germany E-mail:dimhof@uni-bonn.deSearch for more papers by this author Dorle Hennig, Dorle Hennig Center for Molecular Biomedicine, Institute of Biochemistry and Biophysics, Department of Biochemistry, Friedrich Schiller University Jena, Hans-Knöll-Str. 2, D-07745 Jena, GermanySearch for more papers by this authorStephanie Schubert, Stephanie Schubert Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany Department of Pharmaceutical Technology, Friedrich Schiller University, JenaLessingstr. 8, D-07743 Jena, GermanySearch for more papers by this authorHarald Dargatz, Harald Dargatz Institute of Pharmaceutical Biology, Rheinische Friedrich Wilhelms University, BonnNussallee 6, D-53115 Bonn, GermanySearch for more papers by this authorEvi Kostenis, Evi Kostenis Institute of Pharmaceutical Biology, Rheinische Friedrich Wilhelms University, BonnNussallee 6, D-53115 Bonn, GermanySearch for more papers by this authorAlfred Fahr, Alfred Fahr Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany Department of Pharmaceutical Technology, Friedrich Schiller University, JenaLessingstr. 8, D-07743 Jena, GermanySearch for more papers by this authorUlrich S. Schubert, Ulrich S. Schubert Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University, JenaHumboldtstr. 10, D-07743 Jena, GermanySearch for more papers by this authorThorsten Heinzel, Thorsten Heinzel Center for Molecular Biomedicine, Institute of Biochemistry and Biophysics, Department of Biochemistry, Friedrich Schiller University Jena, Hans-Knöll-Str. 2, D-07745 Jena, Germany Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, GermanySearch for more papers by this authorDiana Imhof, Corresponding Author Diana Imhof Pharmaceutical Chemistry I, Institute of Pharmacy, Rheinische Friedrich Wilhelms University, BonnBrühler Str. 7, D-53119 Bonn, Germany E-mail:dimhof@uni-bonn.deSearch for more papers by this author First published: 21 August 2013 https://doi.org/10.1002/mabi.201300213Citations: 9 †Supporting Information is available from the Wiley Online Library or from the author. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The use of different nanoparticles (NPs) for successful encapsulation of bioactive substances is discussed. The inclusion efficiency into liposomes, acetalated dextran (Ac-Dex), and variants of poly[(lactic acid)-co-(glycolic acid)] (PLGA) NPs is analyzed after chemical degradation. Efficient inclusion of SIRT1 inhibitor Ex527 in liposomes, Ac-Dex- and PLGA-NPs is observed for all procedures used. Activity of Ex527 is demonstrated by monitoring the acetylation status of SIRT1-target p53. In contrast, small peptides are only incorporated into acid-terminated PLGA-NPs and marginally into Ac-Dex-NPs. The yield depends on peptide sequence and terminal modifications. Activity is exemplified for angiotensin II using the dynamic mass redistribution technology. Citing Literature Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description mabi201300213-sm-0001-SupTable-S1.pdf126.3 KB Table S1. Chemical characterization data of the peptides described. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume14, Issue1January 2014Pages 69-80 RelatedInformation