DNA‐Mediated Assembly of Gold Nanoparticles and Applications in Bioanalysis

Abstract DNA‐mediated gold nanoparticles (AuNPs) assembly has drawn great attention due to the controllable parameters and properties. In general, DNA sequences working as linkers or templates precisely control the assembly of AuNPs through the Watson–Crick base pairing. Diverse assembly structures show great potential in bioanalysis due to the unique optical characteristics, which can be tuned by the shape, size, composition, particles interactive status and the dielectric constant of the surrounding medium of AuNPs. They exhibit excellent performances for the detection of nucleic acids, proteins, small molecules and other biomarkers. In this review, we present a general overview of the construction of DNA‐mediated AuNP assemblies and the recent advances of these assemblies as high‐performance biosensors. Future directions and major challenges in bioanalysis are also discussed.