美拉德反应
结合
化学
油菜
水溶液
阿拉伯树胶
傅里叶变换红外光谱
纳米颗粒
色谱法
化学工程
有机化学
食品科学
数学
工程类
数学分析
作者
Safoura Pirestani,Ali Nasirpour,Javad Keramat,Stéphane Desobry,Jordane Jasniewski
标识
DOI:10.1016/j.foodhyd.2018.01.001
摘要
We aimed to study the effect of conjugation with gum Arabic (GA) by Maillard reaction in aqueous solution on the structural properties of Canola protein isolate (CPI). First, the grafting of GA to CPI through Maillard reaction was confirmed by examination of the Fourier transform infrared spectra. The results showed that the thermal stability of CPI was remarkably improved by mixing or conjugating with GA. The evidence of particle size distribution determined by nanoparticles tracking analysis (NTA) confirmed that glycation can reduce the thermal aggregation and also nanoparticles of conjugate appear bigger than CPI. Furthermore, the tertiary structure of protein in conjugate changed with decreasing number of aromatic side-chains exposed in heating environment. The decrease in fluorescence intensity of conjugate was related to conformational changes due to presence of GA.
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