牛血清白蛋白
荧光
检出限
四苯乙烯
赫拉
化学
荧光寿命成像显微镜
阳离子聚合
转身(生物化学)
选择性
两亲性
生物物理学
光化学
色谱法
细胞
聚集诱导发射
生物化学
共聚物
有机化学
生物
催化作用
光学
物理
聚合物
作者
Yabei Chen,Ke Li,Shensong Zhang,Pan Xu,Bo Song
标识
DOI:10.1016/j.dyepig.2022.110267
摘要
In this paper, a cationic amphiphile (denoted by DPP-10) with diketopyrrolopyrrole decorated with methoxylated tetraphenylethylene via a phenyl bridge was designed, synthesized, and applied as a turn-on fluorescence probe for bovine serum albumin (BSA) detection and selective cell imaging. DPP-10 showed very weak fluorescence emission when dispersed in the solution, while its emission was drastically enhanced upon the addition of BSA, which should be attributed to the electrostatic interaction-driven assembly between DPP-10 and BSA. The limit of detection was as low as 0.08 μmol/L, which is lower than that reported for most probes. The fluorescence probe shows good selectivity for BSA among the tested proteins, high photostability, and immunity to the disturbance of ions. DPP-10 with excellent cytocompatibility was applied to HeLa cell imaging, where only the BSA-rich cells showed a strong signal-to-background contrast.
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