鲁米诺
电化学发光
纳米复合材料
检出限
材料科学
纳米颗粒
介孔材料
氮化硼
量子点
纳米技术
化学工程
化学
色谱法
有机化学
工程类
催化作用
作者
Yun Tang,Xiao Hu,Yiwei Liu,Yanran Chen,Faqiong Zhao,Baizhao Zeng
标识
DOI:10.1016/j.bios.2022.114492
摘要
A novel electrochemiluminescence (ECL) sensing platform was developed for the detection of neuron-specific enolase (NSE), based on the nanocomposite of mesoporous silica encapsulated CuO2 nanoparticles and electrostatically attracted luminol. An antifouling membrane of polyvinylidene fluoride modified by polyethyleneimine and dopamine was introduced to improve the immobilization of nanocomposite and the stability of ECL signal; Au nanoparticles were loaded on the membrane surface for binding the antibody. The CuO2 nanoparticles were capable of supplying H2O2, while the amino ionic liquid functionalized boron nitride quantum dots as co-reactant of luminol could effectively enhance the ECL signal. The resulting ECL immunosensing platform thus showed excellent performance. Over the concentration range of 5-500 ng/mL, it presented a good linear response; the detection limit was down to 24.5 pg/mL. In addition, it had high selectivity and stability. The sensor has been successfully applied to determine target NSE in human serum samples. This work provides some insights into the further design of high-performance ECL sensors.
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