Combined plasma metabolomic and transcriptomic analysis identify histidine as a biomarker and potential contributor in SLE pathogenesis

发病机制 医学 代谢物 生物标志物 代谢组 免疫学 代谢组学 转录组 免疫系统 内科学 生物信息学 生物 基因表达 基因 遗传学
作者
Yukiko Iwasaki,Yusuke Takeshima,Masaaki Nakano,Masaaki Okubo,Ota M,Akari Suzuki,Yuta Kochi,Tomohisa Okamura,Takaho A. Endo,Ichiro Miki,Kazuhiro Sakurada,Kazuhiko Yamamoto,Keishi Fujio
出处
期刊:Rheumatology [Oxford University Press]
卷期号:62 (2): 905-913 被引量:2
标识
DOI:10.1093/rheumatology/keac338
摘要

Abstract Objectives To investigate metabolite alterations in the plasma of SLE patients to identify novel biomarkers and provide insight into SLE pathogenesis. Methods Patients with SLE (n = 41, discovery cohort and n = 37, replication cohort), healthy controls (n = 30 and n = 29) and patients with RA (n = 19, disease control) were recruited. Metabolic profiles of the plasma samples were analysed using liquid chromatography-time-of-flight mass spectrometry and capillary electrophoresis-time-of-flight mass spectrometry. Transcriptome data was analysed using RNA-sequencing for 18 immune cell subsets. The importance of histidine (His) in plasmablast differentiation was investigated by using mouse splenic B cells. Results We demonstrate that a specific amino acid combination including His can effectively distinguish between SLE patients and healthy controls. Random forest and partial least squares-discriminant analysis identified His as an effective classifier for SLE patients. A decrease in His plasma levels correlated with damage accrual independent of prednisolone dosage and type I IFN signature. The oxidative phosphorylation signature in plasmablasts negatively correlated with His levels. We also showed that plasmablast differentiation induced by innate immune signals was dependent on His. Conclusions Plasma His levels are a potential biomarker for SLE patients and are associated with damage accrual. Our data suggest the importance of His as a pathogenic metabolite in SLE pathogenesis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
ssynkl发布了新的文献求助50
刚刚
2秒前
2秒前
垃圾智造者完成签到,获得积分10
2秒前
樱桃小贩完成签到,获得积分10
3秒前
共享精神应助言言采纳,获得10
3秒前
4秒前
4秒前
zzx完成签到,获得积分10
4秒前
柒八染发布了新的文献求助10
4秒前
斯文明杰发布了新的文献求助10
5秒前
华仔应助zhanks采纳,获得10
5秒前
5秒前
星辰大海应助skskysky采纳,获得10
6秒前
6秒前
哈哈哈发布了新的文献求助10
7秒前
pluto应助淡淡书白采纳,获得10
7秒前
呆桃苏打完成签到,获得积分10
7秒前
桐桐应助金鑫水淼采纳,获得10
8秒前
zwj发布了新的文献求助10
8秒前
745789发布了新的文献求助10
8秒前
freebra发布了新的文献求助10
9秒前
程雯慧发布了新的文献求助10
10秒前
沉默成沨发布了新的文献求助30
10秒前
10秒前
Ava应助TXNM采纳,获得10
10秒前
丘比特应助含糊的幼旋采纳,获得10
10秒前
Joker_Guo发布了新的文献求助10
10秒前
王建国关注了科研通微信公众号
10秒前
所所应助强心健体的酸奶采纳,获得10
11秒前
杨小六发布了新的文献求助10
12秒前
77完成签到 ,获得积分10
13秒前
甄人达发布了新的文献求助10
13秒前
13秒前
奥一奥完成签到,获得积分10
13秒前
英姑应助e746700020采纳,获得10
14秒前
14秒前
zhangsudi发布了新的文献求助10
14秒前
dyj发布了新的文献求助10
15秒前
15秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971125
求助须知:如何正确求助?哪些是违规求助? 3515824
关于积分的说明 11179811
捐赠科研通 3250971
什么是DOI,文献DOI怎么找? 1795610
邀请新用户注册赠送积分活动 875897
科研通“疑难数据库(出版商)”最低求助积分说明 805207