清脆的
Cas9
劈开
DNA
计算生物学
基因组编辑
计算机科学
物理
双股
基因
生物系统
生物
遗传学
DNA修复
作者
Qiao Lu,Deepak Bhat,Darya Stepanenko,Simone Pigolotti
标识
DOI:10.1103/physrevlett.127.208102
摘要
The CRISPR-Cas9 system acts as the prokaryotic immune system and has important applications in gene editing. The protein Cas9 is one of its crucial components. The role of Cas9 is to search for specific target sequences on the DNA and cleave them. In this Letter, we introduce a model of facilitated diffusion for Cas9 and fit its parameters to single-molecule experiments. Our model confirms that Cas9 search for targets by sliding, but shows that its sliding length is rather short. We then investigate how Cas9 explores a long stretch of DNA containing randomly placed targets. We solve this problem by mapping it into the theory of Anderson localization in condensed matter physics. Our theoretical approach rationalizes experimental evidence on the distribution of Cas9 molecules along the DNA.
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