细胞生物学
干细胞
细胞命运测定
生物
再生(生物学)
细胞
骨骼肌
人口
化学
生物化学
内分泌学
基因
转录因子
社会学
人口学
作者
Feng Yue,Stephanie N. Oprescu,Jiamin Qiu,Lijie Gu,Lijia Zhang,Jingjuan Chen,Naagarajan Narayanan,Meng Deng,Shihuan Kuang
出处
期刊:Cell Reports
[Elsevier]
日期:2022-01-01
卷期号:38 (3): 110267-110267
被引量:26
标识
DOI:10.1016/j.celrep.2021.110267
摘要
The lipid droplet (LD) is a central hub for fatty acid metabolism in cells. Here we define the dynamics and explore the role of LDs in skeletal muscle satellite cells (SCs), a stem cell population responsible for muscle regeneration. In newly divided SCs, LDs are unequally distributed in sister cells exhibiting asymmetric cell fates, as the LDLow cell self-renews while the LDHigh cell commits to differentiation. When transplanted into regenerating muscles, LDLow cells outperform LDHigh cells in self-renewal and regeneration in vivo. Pharmacological inhibition of LD biogenesis or genetic inhibition of LD catabolism through knockout of Pnpla2 (encoding ATGL, the rate-limiting enzyme for lipolysis) disrupts cell fate homeostasis and impairs the regenerative capacity of SCs. Dysfunction of Pnpla2-null SCs is associated with energy insufficiency and oxidative stress that can be partially rescued by antioxidant (N-acetylcysteine) treatment. These results establish a direct link between LD dynamics and stem cell fate determination.
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